高效液相色谱-荧光检测法检测植物性食品中14种欧盟优控多环芳烃  

Determination of 14 European Union superior controlled polycyclic aromatic hydrocarbons in plant-orgin foods by high performance liquid chromatography-fluorescence detector

作　　者：路杨[1] 刘印平[1] 王丽英[1] 任贝贝[1] 云鹏[1] 冯静[1] 常凤启[1] LU Yang;LIU Yin-Ping;WANG Li-Ying;REN Bei-Bei;YUN Peng;FENG Jing;CHANG Feng-Qi(Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China)

机构地区：[1]河北省疾病预防控制中心,石家庄050021

出　　处：《食品安全质量检测学报》2020年第18期6594-6600,共7页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱-荧光检测法测定植物性食品如蔬菜、水果及粮食中多环芳烃含量,了解河北省内上述食品中14种欧盟优控多环芳烃的实际污染状况。方法样品经环己烷-乙酸乙酯(1:1,V:V)溶液超声提取后,浓缩至近干,经正己烷复溶后通过多环芳烃专用固相萃取柱进行净化处理,洗脱液经浓缩后用乙腈定容,采用高效液相色谱-荧光检测法进行检测。结果 14种化合物的线性范围为1.00~18.0μg/kg;方法回收率为60.9%~125.4%,相对标准偏差为2.2%~7.6%;苯并(c)芴、苯并(a)蒽、?、5-甲基-1,2-苯并菲、苯并(j)荧蒽、苯并(b)荧蒽、苯并(k)荧蒽、苯并(a)芘、二苯并(a,e)芘、二苯并(a,i)芘、二苯并(a,h)芘检出限为0.15μg/kg;二苯并(a,l)芘、二苯并(a, h)蒽、苯并(g,h,i)苝、茚并(1,2,3-cd)芘检出限为0.30μg/kg。市售的96份样品中,检出不同种类不同含量的多环芳烃类化合物,检出率为51.0%。结论本方法重现性好,检测灵敏度高,可用于水果、蔬菜及粮食中14种多环芳烃的检测。Objective To establish a method for the determination of polycyclic aromatic hydrocarbons(PAHs)in plant-orgin foods including vegetables,fruits and cereal products by high performance liquid chromatography-fluorescence detector(HPLC-FLD),and understand the actual pollution status of the 14 EU-controlled polycyclic aromatic hydrocarbons in the above-mentioned foods in Hebei province.Methods PAHs were extracted by ultrasonic extraction with cyclohexane-ethyl acetate(1:1,V:V),concentrated to nearly dry,redissolved with n-hexane and then purified by special purification column for PAHs.The eluent was concentrated,and the volume was fixed with acetonitrile.The contents of 14 PAHs were determined by HPLC-FLD.Results The linear range of 14 compounds was 1.00-18.0μg/kg.The recovery rate was 60.9%-125.4%,and the relative standard deviation was 2.2%-7.6%.The detection limits of target compounds such as benzo(c)fluorene,benzo(a)anthracene,chrysene,5-methyl chrysene,benzo(j)fluoranthene,benzo(b)fluoranthene,benzo(k)fluoranthene,benzo(a)pyrene,dibenzo(a,e)pyrene,dibenzo(a,i)pyrene and dibenzo(a,h)pyrene were 0.15μg/kg.The detection limits of dibenzo(a,l)pyrene,dibenzo(a,h)fluoranthene,benzo(g,h,i)perylene,indene(1,2,3-cd)pyrene were 0.30μg/kg.A total of 96 samples were collected,and the pollution of PAHs were detected in various foods on the market,and the detection rate was 51.0%.Conclusion The method has good reproducibility and high sensitivity,and can be used for the determination of 14 PAHs in vegetables,fruits and cereal products.

关 键 词：多环芳烃 植物性食品 欧盟 高效液相色谱-荧光检测法 

分 类 号：O657.3[理学—分析化学] TS207.3[理学—化学]