牛至细胞悬浮培养合成多酚及黑曲霉诱导子的促进作用  

Polyphenols Synthesis in Suspension-Cultured Origanum vulgare Cells and Its Stimulation by Aspergillus niger Elicitor

作　　者：李燕平 刘媛 王小强 沈倍韵 周香菊 赵文佳 陈继光[1] 尹忠平[1] LI Yanping;LIU Yuan;WANG Xiaoqiang;SHEN Beiyun;ZHOU Xiangju;ZHAO Wenjia;CHEN Jiguang;YIN Zhongping(Jiangxi Key Laboratory of Natural Products and Functional Food,College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;State Key Laboratory of Medicinal Chemical Biology,Nankai University,Tianjin 300350,China)

机构地区：[1]江西农业大学食品科学与工程学院,江西省天然产物与功能食品重点实验室,江西南昌330045 [2]南开大学药物化学生物学国家重点实验室,天津300350

出　　处：《食品科学》2020年第22期88-96,共9页Food Science

基　　金：国家自然科学基金地区科学基金项目(31260368);天津市自然科学基金项目(15JCZDJC65500);江西省食品药品监督管理局科技项目(2017SP05)。

摘　　要：以牛至细胞悬浮培养合成多酚,通过超高效液相色谱-飞行时间质谱仪检测鉴定该细胞中的多酚类物质,并以外源黑曲霉诱导子促进细胞多酚的合成,研究诱导子质量浓度、添加时间及持续作用时间对细胞生长、细胞形态及多酚合成的影响。结果表明:牛至悬浮培养细胞所合成的多酚类物质主要有原儿茶酸-O-己糖苷、红景天苷、水杨酸-O-葡糖苷、迷迭香酸-3-O-葡糖苷、迷迭香酸、迷迭香酸甲酯6种,以迷迭香酸含量最高,是其中的标志性多酚物质;在细胞指数生长中期(第10天)添加50μg/mL黑曲霉诱导子,持续作用2d,对细胞的生长和形态基本上没有影响,但对细胞合成多酚具有很好的促进效果,迷迭香酸的含量和产量分别提高到对照组的3.25、3.33倍,迷迭香酸产量可达361.38mg/L,总多酚产量增长了1.90倍。研究结果可知,牛至细胞悬浮培养能合成多种多酚,黑曲霉诱导子的诱导能够显著提高该细胞中多酚含量,是提高以迷迭香酸为代表的多酚类化合物产量的有效手段。Origanum vulgare cells were suspension-cultured to synthesize polyphenols,which were further identified by ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry(UPLC-TOF-MS/MS).Aspergillus niger elicitor(ANE)was used to promote polyphenol synthesis in the cultured cells,and the effects of elicitor concentration,addition time and subsequent culture duration on cell growth and polyphenols synthesis were investigated as well.A total of six polyphenols in the cultured cells were identified,including procatechin-O-hexanoside,salidroside,salicylate-Oglucoside,rosmarinic acid-3-O-glucoside,rosmarinic acid(ROA)and methyl rosmarinate,with the most abundant being ROA as a landmark phenolic compound.Adding 50μg/mL ANE at the mid-log phase(the 10th day)hardly affected the growth or morphology of cells cultured for another two days,while it promoted polyphenol synthesis,resulting in a 3.25-fold and 3.33-fold increase in the content and production(reaching 361.38 mg/L)of ROA with a simultaneous 2.90-fold increase in the production of total phenolics.Our results indicated that suspension-cultured O.vulgare cells could synthesize a variety of polyphenols.ANE treatment can effectively enhance the polyphenol content in O.vulgare cells,and therefore,is an effective method to improve the polyphenol yield including ROA as a representative component.

关 键 词：牛至 多酚 迷迭香酸 诱导 

分 类 号：TS201.4[轻工技术与工程—食品科学]