大肠杆菌O157:H7在不锈钢表面生物膜形成能力及其与菌株的特性关系  

Biofilm Formation Ability of Escherichia coli O157:H7 on Stainless Steel Surface and Its Relationship with Bacterial Characteristics

作　　者：吴丽娜 董鹏程[1] 张一敏[1] 毛衍伟[1] 梁荣蓉[1] 杨啸吟[1] 朱立贤[1] 罗欣[1,2] WU Lina;DONG Pengcheng;ZHANG Yimin;MAO Yanwei;LIANG Rongrong;YANG Xiaoyin;ZHU Lixian;LUO Xin(College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China;Jiangsu Collaborative Innovation Center of Meat Production and Processing,Nanjing 210095,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]江苏省肉类生产与加工质量安全控制协同创新中心,江苏南京210095

出　　处：《食品科学》2020年第22期127-132,共6页Food Science

基　　金：现代农业产业技术体系建设专项(CARS-037);山东省现代农业产业技术体系牛产业创新团队项目(SDAIT-09-09);山东省“双一流”奖补资金项目(SYL2017XTTD12)。

摘　　要：以胰蛋白胨大豆肉汤(tryptic soy broth,TSB)和牛肉汁培养基(beef extract,BE)为生长基质,以不锈钢为接触面,探究不同培养基对大肠杆菌O157:H7生物膜形成能力的影响及菌株特性与其生物膜形成能力的相关性。结果表明,菌株、时间、培养基质的交互作用显著影响生物膜的形成(P<0.05)。不同菌株之间的疏水性、自聚能力、泳动能力等特性存在显著差异(P<0.05)。在TSB中,生物膜形成与群体泳动能力存在显著正相关(P<0.05);在BE中,生物膜形成仅与疏水性存在显著正相关(P<0.05)。在本研究中,提供了有关大肠杆菌0157:H7生物膜形成的有用信息,有助于开发有效的生物膜去除技术,并应对牛肉行业中大肠杆菌0157:H7生物膜造成的危害。This study aimed to investigate the effect of two different media:tryptic soy broth(TSB)and beef extract(BE)on the biofilm formation ability of Escherichia coli 0157:H7 on a stainless steel surface and the correlation between the biofilm formation ability and the bacterial characteristics.The results showed biofilm formation capability was significantly influenced by interactions among the strain,the media used,and incubation time(P<0.05).Significant differences in cell surface hydrophobicity,autoaggregation ability and motility were observed among the strains tested(P<0.05).Biofilm formation ability was significantly positively correlated with swarming motility upon culture in TSB(P<0.05)while it was significantly positively correlated with hydrophobicity(P<0.05)upon culture in BE.In addition,there was no correlation of the biofilm formation ability between two culture media of the strains.In conclusion,this study provides useful information for developing effective biofilm removal techniques to control the hazard of E.coli 0157:H7 biofilms in the beef industry.

关 键 词：大肠杆菌O157:H7 生物膜 菌株特性 不锈钢 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]