微波-超声辅助双水相提取藜麦皮皂苷工艺  

The Microwave-Ultrasonic Assisted Aqueous Two-Phase Extraction of Saponins from Chenopodium quinoa Wil d. Bran

作　　者：杨洁[1] 高佳丽 辛燕花 鞠韬 赵二劳[1] YANG Jie;GAO Jiali;XIN Yanhua;JU Tao;ZHAO Erlao(Department of Chemistry,Xinzhou Teachers University,Xinzhou 034000;Department of Biology,Xinzhou Teachers University,Xinzhou 034000)

机构地区：[1]忻州师范学院化学系,忻州034000 [2]忻州师范学院生物系,忻州034000

出　　处：《食品工业》2020年第10期74-79,共6页The Food Industry

基　　金：国家级大学生创新创业训练计划项目(201910124009);山西省应用基础研究计划项目(201901D211458);山西省高等学校科技创新项目(2019L0833);忻州师范学院院级科研项目(201714),忻州师范学院博士科研启动项目(2016)。

摘　　要：以藜麦皮为原料,皂苷提取率为指标,通过单因素试验结合Box-Behnken响应面分析的方法,优化微波-超声辅助双水相提取藜麦皮皂苷的工艺。结果表明,影响藜麦皮皂苷微波-超声辅助双水相提取的因素大小顺序为:加盐量>微波功率>料液比>醇水比值,且加盐(磷酸氢二钾)量对皂苷提取率的影响极显著(p<0.01),料液比和微波功率对藜麦皮皂苷提取率影响显著(p<0.05)。优化的最佳工艺条件为:醇水比值0.75、料液比1∶58 g/mL、加盐(磷酸氢二钾)量3.63 g、微波功率500 W、微波温度40℃、微波时间1.5 min、超声功率400 W、超声温度50℃和超声时间10 min。此工艺条件下,藜麦皮皂苷提取率为5.53%。The microwave-ultrasound assisted aqueous two-phase extraction of saponins from Chenopodium quinoa Willd.bran was studied by Box-Behnken response surface method based on the single experiment with the saponin extraction rate as an indicator.The experimental results showed that the order of factors affecting the extraction of saponin was that the amount of salt>microwave power>solid liquid ratio>alcohol water ratio.The amount of salt(dipotassium hydrogen phosphate)had a significant effect on the extraction rate of saponin(p<0.01),and solid liquid ratio and microwave power had significant effects on the extraction rate(p<0.05).The optimum condition was as follows:alcohol water ratio 0.75,solid liquid ratio 1∶58(g/m L),the amount of salt 3.63 g,microwave power 500 W,microwave temperature 40℃,microwave time 1.5 min,ultrasonic power 400 W,ultrasonic temperature 50℃,and ultrasonic time 10 min.Under this condition,the extraction rate of saponin from Chenopodium quinoa Willd.bran was 5.53%.

关 键 词：藜麦皮 皂苷 微波辅助提取 超声辅助提取 双水相 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]