红紫芽茶花青素合成关键酶活性与重要酚类物质相关性研究  

Study on the Correlation between the Activities of Key Enzymes Involving in Anthocyanin Synthesis and the Contents of Important Polyphenols in Purple Tea

作　　者：曹冰冰 王秋霜[1] 秦丹丹[1] 傅冬和[2] 方开星[1] 姜晓辉[1] 李红建 王青 潘晨东 李波[1] 吴华玲[1] CAO Bingbing;WANG Qiushuang;QIN Dandan;FU Donghe;FANG Kaixing;JIANG Xiaohui;LI Hongjian;WANG Qing;PAN Chendong;LI Bo;WU Hualing(Tea Research Institute,Guangdong Academy of Agricultural Sciences,Guangdong Key Laboratory of Tea Plant Resources Innovation&Utilization Resources Innovation and Utilization,Guangzhou 510640,China;Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]广东省农业科学院茶叶研究所,广东省茶树资源创新利用重点实验室,广东广州510640 [2]湖南农业大学,湖南长沙410128

出　　处：《茶叶科学》2020年第6期724-738,共15页Journal of Tea Science

基　　金：广东省重点领域研发计划(2020B020220004);国家现代农业产业技术体系建设专项(CARS-19);国家自然科学基金(31600550);科技创新战略专项资金(201619TD、R2016YJ-YB3002、R2017PY-QY009、R2018QD-100)。

摘　　要：以广东的红叶1号、红叶2号、丹妃和云南紫娟4种红紫芽品种(系)为供试材料,以英红九号绿芽品种为对照,通过分析酶活性研究了红紫芽茶花青素合成关键酶活性变化规律,并揭示了其与花青素、茶多酚、儿茶素组分等重要酚类物质含量的相互关系。结果表明,红紫芽茶花青素合成过程中,同一季节不同品种(系)类黄酮-3-O-糖基转移酶(UFGT)活性与茶多酚总量和花青素含量均显著正相关,为花青素合成的关键酶;而苯丙氨酸解氨酶(PAL)、查尔酮合成酶(CHS)、类黄酮-3-羟化酶(F3H)、二氢黄酮醇-4-还原酶(DFR)、黄酮醇合成酶(FLS)、花青素合成酶(ANS)和花青素还原酶(ANR)等活性能力与花青素含量变化趋势不存在密切的相关性;春季各样品儿茶素(C)含量与PAL酶活性显著正相关,表儿茶素没食子酸酯(ECG)含量与DFR酶活性存在显著正相关;同一季节不同品种(系)CHS、F3H、ANS以及ANR酶活性与酚类物质含量无显著相关性。In this study,four purple tea cultivars(strains)namely Hongye 1,Hongye 2,Danfei from Guangdong and Zijuan from Yunnan were used as the testing materials.Yinghong 9 with green buds was used as the control.The activity changing patterns of the key enzymes involved in anthocyanin synthesis were studied by the enzyme activities analysis,which revealed the relationship between the enzyme activities and biochemical components in tea.The results show that the activity of flavonoid-3-O-glycosyltransferase(UFGT),a key enzyme involved in anthocyanin synthesis,was positively correlated with the total amount of tea polyphenols and the anthocyanin contents in different samples in the same season.However,the activities of phenylalanine ammonia lyase(PAL),chalcone synthase(CHS),flavonoid-3-hydroxylase(F3H),dihydroflavonol-4-reductase(DFR),flavonol synthase(FLS),anthocyanin synthase(ANS)and anthocyanin reductase(ANR)were not significantly correlated with the anthocyanin contents.There was a significant and positive correlation between catechin(C)content and PAL enzyme activity in spring.Epicatechin gallate(ECG)content and DFR enzyme activity showed the same result.In addition,there was no significant correlation between the activities of CHS,F3H,ANS and ANR enzymes and polyphenols contents in different cultivars(strains)in the same season.

关 键 词：红紫芽茶 花青素 酶 多酚 

分 类 号：S571.1[农业科学—茶叶生产加工]