产高温蛋白酶菌株的筛选及在固态发酵菜籽粕中的初步应用  

Screening of thermophilic protease strains and their preliminary application on rapeseed meal with solid-state fermentation

作　　者：侯小珊 汤颖秀 马海乐[1] 何荣海[1] HOU Xiao-shan;TANG Ying-xiu;MA Hai-le;HE Rong-hai(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212000,Jiangsu,China)

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212000

出　　处：《粮食与油脂》2020年第12期80-84,共5页Cereals & Oils

基　　金：国家重点研发计划(2016YFD0401401);江苏省高校自然科学基金重大项目(17KJA550002);镇江市“1+1+N”新型农业技术推广项目(ZJNJ[2017]03);江苏高校“青蓝工程”资助项目。

摘　　要：以水解圈直径与菌落直径之比(K值)为初筛指标、蛋白酶活力为复筛指标,从菜籽粕中筛选出产高温蛋白酶菌株。RM-2经16S rRNA鉴定后与嗜热脂肪地芽孢杆菌(Geobacillus stearothemophilus)有99%的相似度。当RM-2固态发酵菜籽粕,发酵温度为55℃、料液比为1∶1(g/mL)、发酵时间为24 h时,基质中的多肽得率最高,且显著高于其他较低温度下发酵的多肽得率。The ratio of hydrolysis circle diameter to colony diameter(K value)was used as the primary screening index and the protease activity was used as the re-screening index to screen thermophilic protease strains from rapeseed meal.The similarity between strain RM-2 and Geobacillus stearmophilus was 99% after 16S rRNA identification.When RM-2 was used to ferment rapeseed meal in solid state,the fermentation temperature 55℃,the ratio of material to liquid 1∶1(g/mL)and the fermentation time 24 h,the highest yield of polypeptide was 9.67% which was significantly higher than that of other low temperature fermentation.

关 键 词：高温蛋白酶 嗜热脂肪地芽孢杆菌 固态发酵 多肽 

分 类 号：TS201.3[轻工技术与工程—食品科学]