山西老陈醋源植物乳杆菌173对Ⅱ型糖尿病大鼠的降血糖机制  

Hypoglycemic Mechanism of Lactobacillus plantarum 173 Isolated from Shanxi Aged Vinegar on Type Ⅱ Diabetes Rats

作　　者：许女[1] 郭宏萍 杨光 陈旭峰 张茹 王如福[1] Xu Nü;Guo Hongping;Yang Guang;Chen Xufeng;Zhang Ru;Wang Rufu(College of Food Science and Engineering,Shanxi Agricultural University,Taigu 030801,Shanxi;Center of Experiment and Teaching,Shanxi Agricultural University,Taigu 030801,Shanxi)

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]山西农业大学实验教学中心,山西太谷030801

出　　处：《中国食品学报》2020年第12期131-140,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：山西省重点研发计划项目(201903D221037);山西高粱产业公共支撑平台项目(HZGL202007)。

摘　　要：本文主要研究了山西老陈醋源植物乳杆菌173对Ⅱ型糖尿病大鼠的降血糖机制。试验结果表明菌株173可以显著改善糖尿病大鼠的血糖、血脂和激素水平。荧光定量PCR结果显示植物乳杆菌173可通过调节肝脏PPARs和GLUT4基因的表达,促进葡萄糖稳态和提升胰岛素敏感性。植物乳杆菌173促进了肠道中短链脂肪酸产生菌的生成。短链脂肪酸发挥降糖作用主要是通过活化肠道G-蛋白受体基因(如GPR43,GPR41)进行的。另外,还证明了植物乳杆菌173可以显著上调肠道紧密连结蛋白ZO-1、Claudin、Occludin及黏蛋白MUC2基因的表达,从而增强肠道黏膜屏障功能,发挥降糖功能。Hypoglycemic mechanism of Lactobacillus plantarum 173 isolated from Shanxi aged Vinegar on type Ⅱ diabetes rats was studied in this paper. Results showed that the strain of 173 improved the disorders in blood glucose,lipids, and hormones of diabetes rats. The fluorescence quantitative PCR results suggested that Lactobacillus plantarum173 could regulate liver relative PPARs and GLUT4 mRNA expression, and improved the glucose homeostasis and insulin sensitivity. Moreover, Lactobacillus plantarum 173 promoted the growth of SCFA-producing bacteria. The antidiabetic effects of SCFA were closely associated with intestinal G-protein receptors GPR43 and GPR41. Furtherly, this study proved that the genes(ZO-1, Occludin, Claudin and MUC2) expression related to intestinal mucous membrane barrier were also involved in the beneficial effect of L. plantarum 173 on type Ⅱ diabetes.

关 键 词：植物乳杆菌 糖尿病 

分 类 号：TS201.3[轻工技术与工程—食品科学]