灭菌乳中嗜热脂肪芽孢杆菌商业无菌的最低可视浓度的测定  

Determination of the lowest visible concentration of commercial sterility of Geobacillus stearothermophilus in sterilized milk

作　　者：胡凤月 周燕霞 巩有博 杨亚芳 郭淼 王青龙 张跃川 杨文炼 蔡雪凤 HU Feng-Yue;ZHOU Yan-Xia;GONG You-Bo;YANG Ya-Fang;GUO Miao;WANG Qing-Long;ZHANG Yue-Chuan;YANG Wen-Lian;CAI Xue-Feng(Beijing Food Safety Monitoring and Risk Assessment Center(Beijing Food Inspection Institute),National Food Quality and Safety Inspection and Supervision Center,Beijing 100094,China)

机构地区：[1]北京市食品安全监控和风险评估中心(北京市食品检验所),国家食品质量安全检验监督中心,北京100094

出　　处：《食品安全质量检测学报》2020年第24期9409-9414,共6页Journal of Food Safety and Quality

摘　　要：目的测定灭菌乳中嗜热脂肪芽孢杆菌的商业无菌最低可视浓度。方法随机购买3种不同品牌的灭菌乳,分别添加105、104、103、102、101、100CFU/mL浓度的嗜热脂肪芽孢杆菌制成模拟样品,分别对培养4、7、9、10 d的样品按照GB 4789.26-2013《食品安全国家标准食品微生物学检验商业无菌检验》的检验方法检测。结果培养7d和10d后的3种品牌模拟样品从编号(X-1)-(X-5)(X代表表1中样品信息编号)均可在视野范围内观察到嗜热脂肪芽孢杆菌,同时镜检发现培养10 d后样品中的嗜热脂肪芽孢杆菌并无百倍或百倍以上增殖也无菌落形成芽孢。对培养10 d后的模拟样品进行菌落计数验证以上结论。结论灭菌乳中嗜热脂肪芽孢杆菌商业无菌最低可视浓度为101CFU/mL。灭菌乳中嗜热脂肪芽孢杆菌在培养10d后无明显增殖,也无菌落进入休眠状态。表明灭菌乳中污染嗜热脂肪芽孢杆菌符合商业无菌相对无菌标准。Objective To determine the lowest visible concentration of commercial sterility of Geobacillus stearothermophilus in sterilized milk.Methods Three different brands of sterilized milk were randomly purchased,after adding 10~5,10~4,10~3,10~2,10~1,10~0 CFU/mL concentration Geobacillus stearothermophilus to make a simulated sample,the samples cultured for 4,7,9,and 10 d were tested according to the inspection method of GB 4789.26-2013 National food safety standard-Food microbiological inspection-Commercial sterility inspection.Results After 7 d and 10 d of cultivation,the 3 brand simulation samples from number(X-1)to(X-5)(X represents the sample information number in Table 1)could all be observed in the field of view of Geobacillus stearothermophilus,at the same time,it was found that the Geobacillus stearothermophilus in the sample after 10 days of culture did not multiply a hundred times or more,and did not produce spores.Colony counts were performed on the simulated samples after 10 days of culture to verify the above conclusions.Conclusion The lowest commercial sterile concentration of Geobacillus stearothermophilus in sterilized milk is 10~1 CFU/mL.Geobacillus stearothermophilus in the sterilized milk do not proliferate significantly after 10 days of culture,and no colony enteres a dormant state.It showes that the contaminated Geobacillus stearothermophilus in the sterilized milk meets the commercial sterility relative sterility standard.

关 键 词：灭菌乳 嗜热脂肪芽孢杆菌 商业无菌 检出限 漏检 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]