酶解时间对罗非鱼鱼皮胶原蛋白酶解物抗氧化活性的影响  

Effects of hydrolysis time on antioxidant activity of tilapia skin collagen proteolytic

作　　者：王晴 石程宇 钱玉梅 李红侠 陈红玲 陈嶍嶍 曹稳根 Wang Qing;Shi Chengyu;Qian Yumei;Li Hongxia;Chen Hongling;Cao Wengen;Zang Feng(School of Biology and Food Engineering,Suzhou University,Suzhou 234000 China)

机构地区：[1]宿州学院生物与食品工程学院,安徽宿州234000

出　　处：《湖南文理学院学报（自然科学版）》2021年第1期36-41,共6页Journal of Hunan University of Arts and Science(Science and Technology)

基　　金：安徽省自然科学基金项目(1908085MC100);安徽省教育厅自然科学研究重点项目(KJ2018A0443,KJ2017A-441);安徽省大学生创新训练项目(201910379125);宿州学院学术技术带头人项目(2016XJXS06);宿州学院教授(博士)科研启动基金项目(2016jb02);宿州学院科研平台开放课题(2017ykf05,2019ykf28)。

摘　　要：为了探究酶解时间对罗非鱼鱼皮胶原蛋白酶解物抗氧化活性的影响,采用酸法提取罗非鱼鱼皮胶原蛋白,胃蛋白酶酶解3、6、9、12和24 h制备酶解物,进行紫外光谱扫描,水解度测定,以DPPH·、·OH和O2−·清除率为指标评价抗氧化活性。结果表明:最大紫外吸收峰相似,水解度与酶解时间呈正相关;9 h酶解物对三种自由基清除效果最好;罗非鱼鱼皮胶原蛋白酶解物抗氧化活性与酶解时间有关,为罗非鱼鱼皮进一步加工利用提供理论依据。To investigate the effect of enzymolysis time on the antioxidant activity of collagen protein hydrolysate of tilapia fish skin.Acidic extraction of tilapia fish skin collagen,pepsin enzymolysis for 3,6,9,12 and 24 h to prepare enzymatic hydrolysate,UV spectrum scanning,hydrolysis degree determination,using DPPH,OH and O2−·.The clearance rate is an index to evaluate antioxidant activity.The maximum UV absorption peak was similar,and the degree of hydrolysis was positively correlated with the enzymolysis time.9 h enzyme hydrolysate has the best effect on scavenging three free radicals.Conclusion:The antioxidant activity of collagen proteolytic products of tilapia fish skin is related to the time of enzymolysis,which provides a theoretical basis for further processing and utilization of tilapia fish skin.

关 键 词：罗非鱼鱼皮 胶原蛋白 酶解 抗氧化活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]