一株传统发酵食品来源乳杆菌的分离鉴定及辅助降血糖作用评价  

Isolation and Identification of a Lactobacillus Strain from Traditional Fermented Food and Evaluation of Its Auxiliary Hypoglycemic Effect

作　　者：毕云枫[1] 王善红 赵子健 杨舸 赵玉娟[2] 王超[2] 段翠翠[2] 李盛钰[2] BI Yunfeng;WANG Shanhong;ZHAO Zijian;YANG Ge;ZHAO Yujuan;WANG Chao;DUAN Cuicui;LI Shengyu(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118;Institute of Agrofood Technology,Jilin Academy of Agricultural Sciences,Changchun 130033,China)

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]吉林省农业科学院农产品加工研究所,长春130033

出　　处：《东北农业科学》2020年第6期110-114,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程重大项目(CXGC2017ZD011);国家现代农业(奶牛)产业技术体系建设专项项目(CARS-36);长春市产学研协同创新示范点建设专项项目(16CX20)。

摘　　要：采用API 50 CHL鉴定系统和16S rDNA序列比对方法鉴定菌株。利用高脂饲料结合链脲佐菌素建立小鼠糖尿病模型,通过测定小鼠空腹血糖值、血脂、抗氧化指标、胰岛素等指标,评价菌株CH126的辅助降血糖作用。结果表明:综合API 50 CHL和16S rDNA序列分析结果鉴定菌株CH126为植物乳杆菌(Lactobacillus plantarum)。Ⅱ型糖尿病小鼠灌胃植物乳杆菌CH126后,与模型组相比,CH126组能显著降低小鼠的空腹血糖值、血脂水平,抑制氧化应激损伤,改善肠道屏障功能,提高胰岛素水平。综合以上数据表明植物乳杆菌CH126是具有辅助降血糖功能的益生菌。API 50 CHL identification system and 16 S rDNA sequence alignment method were used to identify the strain. The model of diabetes mellitus in mice was established by high fat diet combined with streptozotocin. The auxiliary hypoglycemic effect of strain CH126 was evaluated by measuring fasting blood glucose, blood lipid, antioxidant index and insulin. The results showed that: the strain CH126 was identified as Lactobacillus plantarum by combining API 50 CHL and 16 S rDNA sequence analysis. Compared with model group, L. plantarum CH126 could significantly reduce fasting blood glucose and blood lipid levels, inhibit oxidative stress injury, improve intestinal barrier function, and increase insulin level. The above data indicate that L. plantarum CH126 is a probiotic with the function of reducing blood sugar.

关 键 词：益生菌 植物乳杆菌CH126 鉴定 降血糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]