草果提取物对大肠杆菌和沙门氏菌抑菌机理研究  

The Antimicrobial Mechanisms of Amomum tsao-ko Extracts Against Escherichia coli and Salmonella typhimurium

作　　者：唐志凌 赵明明 陈靖潼 高璐瑶 陈文学[1] 李远颂[2] TANG Zhi-ling;ZHAO Ming-ming;CHEN Jing-tong;GAO Lu-yao;CHEN Wen-xue;LI Yuan-song(College of Food Science and Engineering,Hainan University,Haikou 570228,China;Graduate Division,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学食品科学与工程学院,海口570228 [2]海南大学研究生处,海口570228

出　　处：《中国调味品》2021年第2期50-54,共5页China Condiment

基　　金：海南省自然科学基金(318MS016);国家自然科学基金项目(31760480)。

摘　　要：文章研究了草果中所含抑菌物质对大肠杆菌和沙门氏菌的抑菌机理。通过最小抑菌浓度(minimum inhibitory concentration,MIC)、最小杀菌浓度(minimum bactericidal concentration,MBC)和生长曲线的测定研究草果抑菌提取物对大肠杆菌和沙门氏菌细胞生长的影响;通过扫描电子显微镜(scanning electron microscopy,SEM)观察细菌细胞微观形态结构的变化;通过测定胞外碱性磷酸酶(alkaline phosphatase,AKP)研究草果抑菌提取物对大肠杆菌和沙门氏菌细胞壁通透性的影响。结果表明,草果提取物对供试菌的生长具有明显抑制作用,大肠杆菌的MIC和MBC值均为0.625mg/mL,沙门氏菌的MIC和MBC值约为1.25mg/mL;草果提取物在作用供试菌8h后,对其细胞结构造成了破坏,使得细胞壁的通透性增加。In this paper,the antimicrobial mechanisms of Amomum tsao-ko extracts against Escherichia coli(E.coli)and Salmonella typhimurium(S.typhimurium)are studied.The effects of Amomum tsao-ko extracts on the growth of E.coli and S.typhimurium are studied by measuring the minimum inhibitory concentration(MIC),the minimum bactericidal concentration(MBC)and the growth curve.The changes of microscopic morphological structure of bacterial cells are observed by scanning electron microscopy(SEM),and the effects of the Amomum tsao-ko extracts on the cell wall permeability of E.coli and S.typhimurium are studied by measuring the alkaline phosphatase(AKP).The results show that Amomum tsao-ko extracts have obvious inhibitory effect on the growth of the tested bacteria,the MIC and MBC values of E.coli are all 0.625 mg/mL,and the MIC and MBC values of S.typhimurium are all 1.25 mg/mL.With the treatment of Amomum tsao-ko extracts for 8 h,the cell structure of E.coli and S.typhimurium is damaged and the permeability of cell wall is increased.

关 键 词：草果提取物 大肠杆菌 沙门氏菌 抑菌机理 扫描电镜(SEM) 碱性磷酸酶(AKP) 

分 类 号：TS201.3[轻工技术与工程—食品科学]