基于Illumina MiSeq测序和传统可培养方法的洪湖鲊广椒乳酸菌多样性研究  

Lactic acid bacterial diversity of Zha-chili in Honghu county based on Illumina Miseq sequencing and traditional culture method

作　　者：李娜 崔梦君 马佳佳 雷炎 郭壮[1] 张振东[1] LI Na;CUI Mengjun;MA Jiajia;LEI Yan;GUO Zhuang;ZHANG Zhendong(Northwest Hubei Research Institute of Traditional Fermented Food,College of Food Science and Technology,Hubei University of Arts and Science,Xiangyang 441053,China)

机构地区：[1]湖北文理学院食品科学技术学院,鄂西北传统发酵食品研究所,湖北襄阳441053

出　　处：《食品与发酵工业》2021年第4期110-115,122,共7页Food and Fermentation Industries

基　　金：湖北文理学院教师科研能力培育基金项目(2017kypy051)。

摘　　要：为明确洪湖鲊广椒的乳酸菌多样性,从湖北省荆州市洪湖市采集了7份鲊广椒样品,分别采用传统可培养方法与Illumina MiSeq高通量技术对其进行多样性评价。高通量测序结果表明,硬壁菌门(Firmicutes)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria)是鲊广椒样品中的优势细菌门,平均相对含量分别为77.78%、18.09%、1.74%和1.51%;优势菌属为乳杆菌属(Lactobacillus),海洋芽孢杆菌属(Oceanobacillus),盐单胞菌属(Halomonas),平均相对含量分别为67.97%,3.88%,3.47%。在分类操作单元(operational taxonomic units,OTU)水平上,有12个平均相对含量>1.0%的OTU,其中9个隶属于乳杆菌属。由此可见,该地区鲊广椒中主要含乳酸杆菌属。基于传统可培养分离方法表明,从鲊广椒样品中分离到的29株乳酸菌被鉴定为3个属和8个种,其中植物乳杆菌(L.plantarum)和福莱乳杆菌(L.futsaii)分别有10株与8株,是优势菌。与其他地区不一样的是,洪湖鲊广椒中除了植物乳杆菌,还含有较多的福莱乳杆菌。Seven samples of Zha-chili were collected from Honghu county,Jingzhou city,Hubei province,to determine their lactic acid bacterial diversity by using Miseq sequencing and traditional culture method.The Miseq sequencing results showed that Firmicutes,Proteobacteria,Bacteroidetes and Actinobacteria were the dominant phylum,and their average relative content were 77.78%,18.09%,1.74%and 1.51%,respectively.The dominant genera were Lactobacillus,Oceanobacillus,and Halomonaas,and their average relative content were 67.97%,3.88%and 3.47%,respectively.At the level of operational taxonomic units(OTUs),a total of 12 OTUs with relative content more than 1.0%were found,of which 9 OTUs belong to Lactobacillus.It was concluded that Lactobacillus was the main bacterium in Zha-chili from Honghu county.The results of traditional culture method showed that 29 Lactobacillus strains isolated from Zha-chili were identified as 3 genera and 8 species,amongst which,Lactobacillus plantarum(10 strains)and Lactobacillus futsaii(8 strains),were the dominant bacterial species.In particular,in addition to L.futsaii,L.plantarum were the main lactic acid bacteria species in Honghu Zha-chili.

关 键 词：鲊广椒 高通量测序 传统可培养方法 乳酸菌 多样性 

分 类 号：TS201.3[轻工技术与工程—食品科学]