Identification of Serum Exosomal MicroRNA Expression Profiling in Menopausal Females with Osteoporosis by High-throughput Sequencing  

作　　者：Jian-li SHAO Heng LI Xiao-rong ZHANG Xia ZHANG Zhi-zhong LI Gen-long JIAO Guo-dong SUN 

机构地区：[1]Department of Orthopedics and Traumatology,First Affiliated Hospital,Jinan University,Guangzhou 510632,China [2]Department of Orthopedics,Centred People’s Hospital of Zhanjiang,Zhanjiang 524045,China [3]Department of Ultrasound,Mental Hospital of Civil Affairs Bureau,Guangzhou 510632,China

出　　处：《Current Medical Science》2020年第6期1161-1169,共9页当代医学科学（英文）

基　　金：This project was supported by grants from National Natural Science Foundation of China(No.31970862);Natural Science Foundation of Guangdong Province(No.2018A030313576 and No.2019A1515011335).

摘　　要：Estrogen deficiency,which mainly occurs in postmenopausal women,is a primary reason for osteoporosis in clinical diagnosis.However,the molecular regulation of osteoporosis in menopausal females is still not adequately explained in the literature,with the diagnosis and treatment for osteoporosis being limited.Herein,exosomal microRNAs(miRNAs)were used to evaluate their diagnosis and prediction effects in menopausal females with osteoporosis.In this study,6 menopausal females without osteoporosis and 12 menopausal females with osteoporosis were enrolled.The serum exosomes were isolated,and the miRNA expression was detected by miRNA high-throughput sequencing.Exosomal miRNA effects were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses.The miRNA-targeted genes were evaluated by Targetscan 7.2 and the protein-protein interactions(PPI)by STRING.Hub genes were analyzed by the CytoHubba app of Cytoscape.The results showed that 191 aberrant miRNAs were found in the group of menopausal females with osteoporosis,including 72 upregulated miRNAs and 121 downregulated miRNAs.Aberrant miRNAs were involved in many signaling pathways,such as the Wnt,MAPK,and Hippo pathways.Based on PPI network analysis,FBXL3,FBXL13,COPS2,UBE2D3,DCUN1D1,DCUN1D4,CUL3,FBXO22,ASB6,and COMMD2 were the 10 most notable genes in the PPI network.In conclusion,aberrant serum exosomal miRNAs were associated with an altered risk of osteoporosis in menopausal females and may act as potential biomarkers for the prediction of risk of osteoporosis in menopausal females.

关 键 词：EXOSOME diagnosis BIOMARKER OSTEOPOROSIS 

分 类 号：R73[医药卫生—肿瘤]