绿茶面包中儿茶素总量检测方法研究  

Determination of Total Catechins in Green Tea Bread

作　　者：宋振硕[1] 张磊[1] 王丽丽[1] 林清霞 陈林[1] SONG Zhen-shuo;ZHANG Lei;WANG Li-li;LIN Qing-xia;CHEN Lin(Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China)

机构地区：[1]福建省农业科学院茶叶研究所,福建福州350013

出　　处：《茶叶学报》2021年第1期7-10,共4页Acta Tea Sinica

基　　金：福建省属公益类科研院所基本科研专项(2017R1012-3);福建省农业科学院对外合作项目(DEC201821216)。

摘　　要：儿茶素是茶叶的主要功能成分,本试验探讨了乙醇浸提、硫酸-香荚兰素比色法检测绿茶面包中儿茶素含量的可行性。选择95%乙醇溶液作为提取溶剂,采用3因素3水平正交试验设计方案,考察料液比、浸提温度、浸提时间对绿茶面包中儿茶素总量提取效果的影响,并对硫酸-香荚兰素比色法检测绿茶面包中儿茶素含量进行了方法学考察。结果表明,绿茶面包中儿茶素总量检测的最佳浸提条件为:料液比1∶20(g·mL^(-1)),70℃水浴浸提45 min;硫酸-香荚兰素比色方法为:取0.5 mL浸提液,加入2.5 mL1%的香荚兰素/乙醇溶液和2.5 mL30%的硫酸/乙醇溶液,在20℃下反应10~30 min,取反应液在500 nm处测定吸光度。本方法中儿茶素浓度在0.025~0.3 mg·mL^(-1)范围内,吸光度与浓度线性关系良好(R^(2)=0.9996),同一样品重复测定6次的RSD为1.31%,儿茶素高、中、低浓度加标回收率范围在98.75%~103.25%,本方法满足绿茶面包中儿茶素总量的检测分析,儿茶素含量可作为标定绿茶面包中茶叶添加量的评价指标。An assay method was established for the determination of total catechins,the major functional component of tea,in bread made with ultrafine green tea powder as an ingredient.The method was optimized with a 3-factor(i.e.,solid-liquid ratio,extraction temperature,and extraction time)and 3-level orthogonal experiment for the 95%ethanol extraction prior to a colorimetric measurement after the sulfuric acid-vanillin reaction.The optimized ethanol extraction was conducted under a solid-liquid ratio of 1:20(g·mL^(-1))in a 70℃water bath for 45m.Subsequently,0.5 mL of the extract were added with 2.5 mL each of 1%vanillin and 30%sulfuric acid ethanol solutions to stand at 20℃for 10~30 min.Spectrophotometric absorbance at 500 nm of the reaction solution was obtained.The readings were found correlated linearly with the standard catechins concentrations in the range of 0.025~0.3 mg·mL^(-1) with a R^(2) of 0.9996.The RSD of 6 replicates measured by the new method was 1.31%.And,the catechins recovery at high-to-low concentrations ranged from 98.75%to 103.25%.The method appeared appropriate for the total catechins determination in bread containing green tea.

关 键 词：超微绿茶粉 面包 儿茶素 香荚兰素 

分 类 号：TS272.2[农业科学—茶叶生产加工]