假单胞菌噬菌体PrH-181对大黄鱼块防腐效果的研究  

Antiseptic Effect of Pseudomonas Bacteriophages PrH-181 on Large Yellow Croaker Meat

作　　者：胡申才[1] 楚乐乐 周敏 HU Shencai;CHU Lele;ZHOU Min(School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Hubei Province Fresh Food Engineering Research Center,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生物制药工程学院,湖北武汉430023 [2]湖北省生鲜食品工程技术研究中心,湖北武汉430023

出　　处：《食品工业科技》2021年第6期288-291,324,共5页Science and Technology of Food Industry

基　　金：国家重点研发项目(2017YFC1600100)。

摘　　要：为控制大黄鱼块中假单胞菌引起的腐败作用,本研究测试了1株假单胞菌裂解性噬菌体PrH-181在4℃有氧冷藏条件下对大黄鱼的防腐作用效果。经10^(2)~10^(8) pfu/mL范围内不同效价噬菌体处理无菌大黄鱼汁后30℃培养至14 h,OD_(620nm)测定结果显示,噬菌体效价大于10^(3) pfu/mL时就具有显著(P<0.05)的抑菌活性。从鱼块菌落数、pH和TVB-N含量等腐败临界关键指标变化来看,对照组中的假单胞菌落数在第4 d时就达到了临界值10^(6) cfu/g,pH在第2 d时就降到了最低点,TVB-N含量在第4 d时已经超过临界值30 mg/100 g;而对10^(4)和10^(6) pfu/mL的噬菌体处理组来说,假单胞菌落数分别于第10和12 d才达到临界值10^(6) cfu/g,最低pH出现时间分别为第8和12 d,TVB-N含量分别于第12和14 d才超过临界值。可见噬菌体PrH-181对大黄鱼块的保鲜效果明显,且效价越高保鲜效果越好。In order to control the spoilage of raw large yellow croaker fillets caused by Pseudomonas.The antibacterial ability of the Pseudomonas bacteriophage PrH-181 against the spoilage of large yellow croaker under aerobic refrigeration at 4℃was studied.After treating the fish juice with different titer of 10^(2) to 10^(8) pfu/mL,incubating for 14 h at 30℃,the results of OD_(620nm) showed the bacteriophage PrH-181 exhibited significant(P<0.05)antibacterial activity,when its titer was greater than 10^(3) pfu/mL.From the change of colony numbers,pH and TVB-N concentration which characterize the extent of spoilage,as for the control,there were 10^(6) cfu/g Pseudomonas,which was the critical value as symbolic of spoilage beginning,in the fourth day,and the minimun pH occurred in the second day,and the TVB-N concentration exceeded the critical value 30 mg/100 g on the fourth day.As for 10^(4) pfu/mL and 10^(6) pfu/mL phage-treated group,the time for the critical value of colony numbers occurred on the 10th and 12th day,and minimum pH occurred on the 8th and 12th day,TVB-N concentration was up to the critical value on the 12th and 14th day.So,it could be inferred that the preservation effect of bacteriophage PrH-181 on the large yellow croaker was obvious,and the higher the titer was,the better the preservation effect would be.

关 键 词：大黄鱼 腐败控制 假单胞菌 假单胞菌裂解性噬菌体PrH-181 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]