响应面法优化超声辅助提取梵净山薇菜多酚  

Optimization of ultrasonic assisted extraction of Osmunda japonica polyphenols in Mount Fanjing by response surface method

作　　者：杨天友 王琴[1] 赵芮 李刚凤 YANG Tian-you;WANG Qin;ZHAO Rui;LI Gang-feng(College of Agroforestry Engineering and Planning,Tongren University,Tongren 554300,Guizhou,China;Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region,Tongren University,Tongren 554300,Guizhou,China;College of Material and Chemical Engineering,Tongren University,Tongren 554300,Guizhou,China)

机构地区：[1]铜仁学院农林工程与规划学院,贵州铜仁554300 [2]铜仁学院贵州省梵净山地区生物多样性保护与利用重点实验室,贵州铜仁554300 [3]铜仁学院材料与化学工程学院,贵州铜仁554300

出　　处：《粮食与油脂》2021年第3期111-114,132,共5页Cereals & Oils

基　　金：铜仁学院学科建设与研究生教育专项项目(trxkm[2018]12);贵州省环保厅科学研究课题资助项目(财建[2014]348);贵州省科技厅联合基金项目(黔科合L H字[2014]7477);贵州省重点实验室项目(黔科合平台人才[2020]2003号);铜仁市科技局科技支撑计划项目(铜市科研[2020]128号)。

摘　　要：以梵净山四级薇菜干为原料,研究超声辅助提取梵净山薇菜多酚最佳工艺条件。在单因素试验的基础上,选择超声温度、超声时间、乙醇体积分数、超声功率为关键因素,以薇菜多酚得率为响应值,设计Box-Behnken响应面法优化其超声提取条件。结果表明:各因素对梵净山薇菜多酚得率的影响程度从大到小依次为料液比﹥乙醇体积分数﹥超声时间﹥超声功率,薇菜多酚的最佳提取工艺参数为料液比1∶30(g/mL)、乙醇体积分数40%、超声功率300 W、超声时间40 min。在此条件下,梵净山薇菜多酚得率预测值为38.5967%,试验测定值为38.57%,与回归方程的理论预测值相吻合。Four-level Osmunda japonica from Fanjing Mountain was used as raw material,the ultrasonicassisted extraction of polyphenols from the dried Osmunda japonica from Fanjing Mountain was studied.On the basis of single factor experiments,ultrasonic temperature,ultrasonic time,ethanol volume fraction and ultrasonic power were selected as the key factors,and Box-Behnken response surface methodology was designed to optimize the ultrasonic extraction conditions of Osmunda japonica polyphenols taking the yield of Osmunda japonica polyphends as the response value.The results showed that the effects of various factors on the polyphenols yield of Osmunda japonica from Fanjing Mountain were as follows:solid-liquid ratio>ethanol volume fraction>ultrasonic time>ultrasonic power.The optimal extraction parameters of Osmunda japonica polyphenols were as follows:solid-liquid ratio 1∶30(g/mL),ethanol volume fraction 40%,ultrasonic power 300 W and ultrasonic time 40 min.Under these conditions,the predicted value of polyphenols yield of Osmunda japonica from Fanjing Mountain was 38.5967%,and the measured value was 38.57%,which was consistent with the theoretical prediction value of regression equation.

关 键 词：薇菜 多酚 超声波 响应面法 

分 类 号：TS201.1[轻工技术与工程—食品科学]