高产β-葡聚糖的黄伞菌株分离、鉴定及其体外模拟消化  

Isolation,identification and in vitro simulated digestion of Pholiota adiposa with a high production ofβ-glucan

作　　者：韩宛芸 张长懿 顾泽鹏 段小雨 孙庆杰[2] 邱立忠 卞希良 邬应龙[1] 刘韫滔[1] HAN Wanyun;ZHANG Changyi;GU Zepeng;DUAN Xiaoyu;SUN Qingjie;QIU Lizhong;BIAN Xiliang;WU Yinglong;LIU Yuntao(College of Food Science,Sichuan Agricultural University,Ya’an 625014,China;College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Zhucheng Xingmao Corn Development Co.Ltd.,Weifang 261000,China)

机构地区：[1]四川农业大学食品学院,四川雅安625014 [2]青岛农业大学食品科学与工程学院,山东青岛266109 [3]诸城兴贸玉米开发有限公司,山东潍坊261000

出　　处：《食品与发酵工业》2021年第7期51-57,共7页Food and Fermentation Industries

基　　金：四川省应用基础研究计划(2020YJ0156);四川省科技创新苗子工程项目(2020JDRC0085);四川省重点研发项目(2019YFN0176)。

摘　　要：分离、纯化获得1株高产β-葡聚糖的黄伞菌株PA-008,为进一步提高该菌株中β-葡聚糖的产量和产率,通过单因素和正交试验,对培养基成分及液态发酵条件进行优化。优化得到的发酵条件为:碳源为葡萄糖,氮源为酵母浸出粉,发酵温度25℃,初始pH值6.0。同时,采用体外消化模型对黄伞菌丝体中主要营养物质(氨基酸、矿物质、β-葡聚糖)的生物可利用率进行分析。结果表明,黄伞菌丝体中的氨基酸及矿物质均有一定程度的释放,且重金属矿物质(Pb、Cd、As)经体外模拟消化后均低于安全值,消化液中β-葡聚糖含量为41.92 mg/g,生物可利用率达15.50%,实验所获数据为更科学和深入地评估黄伞菌株提供了理论基础。A Pholiota adiposa strain PA-008 with a highβ-glucan-producing property(GenBank accession number:MF687867)was obtained by isolation and purification.In order to further improve the production and yield ofβ-glucan of strain,single-factor and orthogonal experiments were carried out to design and optimize the medium composition and liquid fermentation conditions of PA-008.The results showed that the optimal fermentation conditions of the strains of PA-008 were as follows:glucose,yeast powder,fermentation temperature 25℃,initial pH value 6.0.Meanwhile,an in vitro digestion model was used to analyze the bioavailability of the main nutrients(amino acids,minerals,andβ-glucans)in the P.adiposa.The results showed that there was a certain degree of amino acids and minerals release in the mycelium of the P.adiposa.And the release of heavy metal minerals(Pb,Cd,As)were lower than the safe value after in vitro simulated digestion.Theβ-glucose content in the digestive juice was 41.92 mg/g,and its bioavailability reached 15.50%.The above data provided a theoretical basis for a more scientific and in-depth evaluation of the P.adiposa.

关 键 词：黄伞菌株 Β-葡聚糖 液态发酵 体外模拟消化 生物可利用率 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]