气相色谱法测定调和油中18种脂肪酸  

Determination of 18 fatty acids in mixed oil by gas chromatography

作　　者：王延华[1] 范荣波 周霞[1] 殷兰兰[1] WANG Yan-Hua;FAN Rong-Bo;ZHOU Xia;YIN Lan-Lan(Haidu College,Qingdao Agricultural University,Yantai 265200,China)

机构地区：[1]青岛农业大学海都学院,烟台265200

出　　处：《食品安全质量检测学报》2021年第5期1978-1981,共4页Journal of Food Safety and Quality

基　　金：基于应用型人才培养的食品营养学课程的教学改革项目(JY201916)。

摘　　要：目的建立气相色谱法测定调和油中18种脂肪酸的方法。方法样品经过氢氧化钾甲醇溶液甲酯化,采用毛细管色谱柱(100 mm×0.25 mm, 0.2μm)和程序升温模式进行测定,检测器温度为280℃。结果 18种脂肪酸的线性关系良好(r>0.999),检出限为0.0005~0.009mg/kg,回收率为78.9%~99.1%,相对标准偏差(relative standard deviation, RSD)为0.768%~8.236%。结论该方法快速、简单、回收率和精密度良好,可用于调和油中脂肪酸的的检测。Objective To establish a method for the determination of 18 fatty acids in blending oil by gas chromatography.Methods The sample was esterified with potassium hydroxide methanol solution,and the capillary column(100 mm×0.25 mm,0.2μm)and temperature programmed mode were used.The detector was hydrogen flame ion detector at 280℃.Results The linear relationship of 18 fatty acids was good(r>0.999),the detection limits of 18 fatty acids were 0.0005‒0.009 mg/kg,the recoveries were 78.9%‒99.1%,relative standard deviations(RSD)were 0.768%‒8.236%.Conclusion The method is rapid,simple,with good recovery and precision,and can be used for the determination of fatty acids in oil.

关 键 词：调和油 脂肪酸 气相色谱法 

分 类 号：O657.71[理学—分析化学] TS225.1[理学—化学]