圆铃1号枣多酚的提取及抗氧化活性分析  

Extraction and Antioxidant Activities of Polyphenols from Zizyphus jujuba cv. Yuanling 1

作　　者：张砚垒 高琳[1,2] 付亚玲 杨燕敏 张仁堂 ZHANG Yan-lei;GAO Lin;FU Ya-ling;YANG Yan-min;ZHANG Ren-tang(College of Food Science and Engineering,Shandong Agricultural University,Taian 271018,Shandong,China;Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,Taian 271018,Shandong,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]山东省高校食品加工技术与质量控制重点实验室,山东泰安271018

出　　处：《食品研究与开发》2021年第8期49-55,共7页Food Research and Development

基　　金：山东省重点研发计划项目(2016GNC113015);山东省2017年度农业重大应用技术创新项目(SDNY2017006);2019年度山东省重点研发计划(公益类专项)(2019GNC106061);佳县红润枣业专业合作社横向课题(SDAUHX2018009)。

摘　　要：以圆铃1号枣为试验材料,通过单因素和正交试验优化其多酚的提取条件,并对其酚酸组成、体外抗氧化能力进行初步研究。结果表明:乙醇体积分数为60%、提取温度为60℃、料液比1∶16(g/mL)、提取时间20 min时,多酚提取量最大,达到(10.69±0.078)mg/g。采用高效液相色谱法鉴定出6种酚酸,分别为芦丁、香豆素、咖啡酸、肉桂酸、(+)-儿茶素、绿原酸,其中芦丁的含量最高,为(17.09±0.48)mg/100 g DW。其多酚提取物的DPPH·清除能力为(17.96±0.95)mg Trolox/g、ABTS+·清除能力为(27.79±3.06)mg Trolox/g、亚铁离子还原力为(32.89±2.17)mg Trolox/g,是一种极具潜力的天然抗氧化剂。The extraction conditions of polyphenols from‘Yuanling 1’jujube was optimized by single factor and orthogonal tests,and the phenolic acid composition and antioxidant capacity of the polyphenols were preliminarily studied. The results showed that the extraction of polyphenols reached(10.69 ± 0.078)mg/g when the volume fraction of ethanol was 60%,the extraction temperature was 60 ℃,the solid-liquid ratio was 1∶16(g/mL)and the extraction time was 20 min. Six phenolic acids were identified by high performance liquid chromatography(HPLC):rutin,coumarin,caffeic acid,cinnamic acid,(+)-catechin and chlorogenic acid,among which rutin content was the highest(17.09 ± 0.48)mg/100 g DW. The DPPH·scavenging capacity of polyphenol extract was(17.96 ± 0.95)mg Trolox/g,ABTS+·scavenging capacity was(27.79 ± 3.06)mg Trolox/g,and the ferric ion reducing capacity(FRAP)was(32.89 ± 2.17)mg Trolox/g. It is a natural antioxidant with great potential.

关 键 词：枣 多酚 提取工艺 酚酸 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]