复合酶法制备紫花芸豆抗氧化肽  

Preparation of antioxidant peptide from kidney bean by complex enzyme method

作　　者：李雪[1] 左锋[1] 王长远[1,2] LI Xue;ZUO Feng;WANG Chang-yuan(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang Province,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江省农产品加工与质量安全重点实验室,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2021年第3期349-356,共8页Chinese Journal of Biologicals

基　　金：国家重点研发计划项目(2017YFD0401203);黑龙江省自然基金项目(LH2019C054);黑龙江省农垦总局农业技术试验示范专项(HNK135-05-01);食品营养及生物活性物质的健康功能作用研究(2017YFA0400200)。

摘　　要：目的采用复合酶法制备紫花芸豆蛋白抗氧化肽。方法以水解度为指标选定碱性蛋白酶和中性蛋白酶为复合酶组合,在单因素试验基础上,进行复合酶水解紫花芸豆蛋白的响应面优化。结果最适水解条件为:温度55℃,pH 10,酶添加比例为1∶2(碱性蛋白酶∶中性蛋白酶),酶添加量5 000 u/mL,在此条件下,紫花芸豆蛋白的水解度为42.2%,与理论值相差较小。对水解后的芸豆蛋白肽进行抗氧化性测定,羟自由基和DPPH清除率分别达37.5%和40.1%,具有较强的抗氧化性。结论复合酶法制备紫花芸豆蛋白抗氧化肽用时短、效率较高,为紫花芸豆抗氧化肽的制备提供参考,也为紫花芸豆蛋白肽在天然抗氧化剂开发方面提供技术支持。Objective To prepare antioxidant peptide from kidney bean by complex enzyme. Methods Alkaline protease and neutral protease were selected as the composite enzymes using the degree of hydrolysis as an indicator.Based on the single factor experiment,the response surface optimization of the complex enzyme hydrolysis of kidney bean protein was carried out. Results The condition for hydrolysis was optimized as follows: temperature 55 ℃,pH 10,enzyme addition ratio(alkaline protease∶neutral protease)1∶2,and enzyme addition amount 5 000 u/mL. Under the condition,the degree of hydrolysis of kidney bean protein was 42. 2%,which was insignificantly different from the theoretical value. The antioxidant activity of hydrolyzed kidney bean protein was determined. The result showed that the removal rate of hydroxyl radical and DPPH were 37. 5% and 40. 1%,respectively,indicating strong antioxidant activity.Conclusion The complex enzyme method for preparation of antioxidant peptide from kidney bean was time-saving and effective,which provided a reference for preparation of antioxidant peptides from kidney bean and a technical support for application of antioxidant peptide in the development of natural antioxidants.

关 键 词：复合酶法 芸豆蛋白 抗氧化肽 响应面 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]