双水相体系超声萃取螺旋藻中β-胡萝卜素及其抗糖基化作用  

Ultrasonic Extraction ofβ-Carotene from Spirulina platensis in Aqueous Two-phase System and Its Anti-glycosylation Effect

作　　者：王音[1] 张喜峰 罗光宏 WANG Yin;ZHANG Xifeng;LUO Guanghong(Faculty of Science,Linfen College of Shanxi Normal University,Linfen 041000,China;Gansu Engineering Technology Research Center for Microalgae,Hexi University,Zhangye 734000,China)

机构地区：[1]山西师范大学临汾学院自然科学系,山西临汾041000 [2]河西学院,甘肃省微藻工程技术研究中心,甘肃张掖734000

出　　处：《食品工业科技》2021年第8期152-157,共6页Science and Technology of Food Industry

基　　金：省创新中心建设项目(甘肃省科技计划资助18JR2JG001)。

摘　　要：研究超声辅助有机溶剂/糖双水相体系萃取螺旋藻中β-胡萝卜素最佳提取条件,并对其抗糖基化作用进行分析,在确定萃取体系为叔丁醇/麦芽糖双水相体系基础上,以螺旋藻粉末加入量、超声时间、超声功率为自变量,β-胡萝卜素得率为因变量,采用正交试验设计优化萃取条件。采用赖氨酸-乳糖模拟体系评价萃取后β-胡萝卜素抗糖基化能力。结果表明:3.4 g叔丁醇/2.4 g麦芽糖体系中,螺旋藻粉末加入量为0.05 g,补足水分至10 g,在超声功率90 W,超声时间5 min条件下,螺旋藻中β-胡萝卜素萃取得率为3.19 mg/g,在浓度为50~450μg/mL范围内,其对模拟体系形成的晚期糖基化终产物的最高抑制率为47.28%,可为新型晚期糖基化终末产物(AGEs)抑制剂开发提供参考依据。The optimum extraction conditions ofβ-carotene from Spirulina platensis by ultrasonic assisted organic solvent/sugar aqueous two-phase system were studied,and its anti glycosylation effect was analyzed.On the basis of determining the extraction system as tert butyl alcohol/maltose aqueous two-phase system,the amount of Spirulina platensis powder,ultrasonic time and ultrasonic power were taken as independent variables,and the yield ofβ-carotene was taken as dependent variable.The results showed that,in the system of 3.4 g tert-butanol/2.4 g maltose,the amount of Spirulina powder was 0.05 g,fill up with water to 10g,under the ultrasonic power of 90 W and ultrasonic time of 5 min,the extraction yield ofβ-carotene from Spirulina was 3.19 mg/g.Whenβ-carotene concentration was at ranges of 50 to 450μg/mL,the highest inhibition rate of AGEs was 47.28%,which could provide a reference for the development of novel AGEs inhibitors.

关 键 词：Β-胡萝卜素 螺旋藻 超声波萃取 叔丁醇/麦芽糖体系 抗糖基化 

分 类 号：TS201.1[轻工技术与工程—食品科学]