茯砖茶金花发酵剂的制备工艺优化  

Optimization on Preparation Technology for Fuzhuan Brick Tea Golden Flower Fermentation Agent

作　　者：曹霞 刘永翔[2] 黄永会[2] 任锡毅 邹晓 谭玉梅[2] CAO Xia;LIU Yongxiang;HUANG Yonghui;REN Xiyi;ZOU Xiao;TAN Yumei(Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering(CICMEAB),College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang,Guizhou 550025;Guizhou Key Laboratory of Agricultural Biotechnology,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与保护种质创新教育部重点实验室,山地生态与农业生物工程协同创新中心,贵州贵阳550025 [2]贵州省农业生物技术重点实验室,贵州贵阳550006

出　　处：《贵州农业科学》2021年第2期140-145,共6页Guizhou Agricultural Sciences

基　　金：贵州省科技支撑计划项目[黔科合支撑(2017)2561];贵州省农业科学院专项(GAAS-SP-2014/004)。

摘　　要：【目的】对茯砖茶金花发酵剂的制备工艺进行优化,为茯砖茶的工艺改良和品质提升提供参考。【方法】以发酵剂产孢量为指标,通过单因素试验和响应面试验优化人工接种金花菌发酵剂配方。【结果】茯砖茶金花发酵剂的最佳制备工艺为以10 g黑毛茶为基质,添加含水量31.96%、蔗糖含量2.98%、接种量321.92μL,28℃条件下,该条件下培养10 d的产孢量达27.36×107个/g。【结论】优化的工艺参数经济可行,孢子产量更高及发花品质较好,更加适宜进行工厂化推广应用。【Objective】The preparation technology for Fuzhuan Brick Tea golden flower fermentation agent is optimized,which provide a reference for technology and quality improvement of Fuzhuan Brick Tea.【Method】Taking spore production of fermentation agent as indicator,single factor and response surface experiments are used to optimize the formula of artificial inoculating golden flower bacteria fermentation agent.【Result】The optimum preparation technology for Fuzhuan Brick Tea golden flower fermentation agent includes 10 g raw dark tea as substrate,adding 31.96%water content,2.98%sucrose content and 321.92μL inoculum dosage and at 28℃.The spore production is up to 27.36×107/g under the optimum condition for culture 10 d.【Conclusion】The optimized technology parameter is economical and practical,the spore production is higher and flower quality is better.It can be more suitable for factory promotion and application.

关 键 词：茯砖茶 金花发酵剂 制备工艺 

分 类 号：S571.1[农业科学—茶叶生产加工] Q939.9[农业科学—作物学]