儿茶素对巨噬-脂肪细胞共培养体系中细胞因子基因表达及AMPK/SIRT1磷酸化水平的影响  

Effect of Catechin on the Gene Expression of Cytokines and Phosphorylation Level of AMPK/SIRT1 in Macrophage and Adipocytes Co-cultured System

作　　者：程安玮 张梦迪[2,3] 王厚伟 孙金月[2] 王新坤[2] 刘超[2] 王青[2] 郭溆[2] Cheng Anwei;Zhang Mengdi;Wang Houwei;Sun Jinyue;Wang Xinkun;Liu Chao;Wang Qing;Guo Xu(College of Food Science and Technology,Hunan Agricultural University,Changsha 410128;Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs,Key Laboratory of Agro-Products Processing Technology of Shandong Province,Institute of Agro-Food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 2501003;College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355)

机构地区：[1]湖南农业大学食品科学技术学院,长沙410128 [2]山东省农业科学院农产品研究所,农业农村部新食品资源加工重点实验室,山东省农产品精深加工技术重点实验室,济南250100 [3]山东中医药大学药学院,济南250355

出　　处：《中国食品学报》2021年第4期64-70,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：山东省农业重大应用技术创新项目(SD2019ZZ016);湖南省教育厅专项(19A236);山东省泰山学者人才工程项目(tshw20120747)。

摘　　要：基于巨噬-脂肪细胞共培养体系研究儿茶素对细胞炎性因子、炎性酶基因表达及AMPK、SIRT1磷酸化水平的影响,初步阐明儿茶素的抗炎作用机制。儿茶素的添加质量浓度为0,50,100μg/mL,采用定量PCR方法检测细胞炎性因子及炎性酶基因的表达水平,采用Western blotting方法检测AMPK及SIRT1的磷酸化水平。结果表明,各处理组中,儿茶素明显抑制促炎性因子(IL-1、IL-6和IL-12)及炎性酶(iNOS和COX-2)基因的表达,促进抗炎性因子(IL-4和IL-10)基因的表达,并且提高AMPK和SIRT1的磷酸化水平,AMPK和SIRT1两者之间呈协同作用。共培养体系中巨噬细胞TNF-α的分泌可诱导脂肪细胞的分化与成熟,儿茶素通过AMPK/SIRT1信号途径调节细胞炎性因子基因的表达实现其抗炎作用。Based on the macrophage and adipocytes co-cultured system,effect of catechins on the gene expression of cytokines and inflammatory enzymes,the phosphorylation levels of AMPK and SIRT1 was studied,and to preliminarily research the anti-inflammatory mechanism of catechin.Catechin with the mass concentrations of 0,50,100μg/mL were added to the four treatments,respectively.The gene expression of cytokines and inflammatory enzymes,phosphorylation levels of AMPK and SIRT1 in adipocytes were determined by quantitative RT-PCR and Western blotting,respectively.Catechin obviously inhibited the gene expression level of pro-inflammatory cytokines(IL-1,IL-6,IL-12)and inflammatory enzymes(iNOS,COX-2),but promoted the expression of anti-inflammatory cytokines(IL-4,IL-10).High mass concentration of catechin increased the phosphorylation level of AMPK and SIRT1,it is synergistic between AMPK and SIRT1.In the macrophage and adipocytes co-cultured system,the secretion of TNF-αin macrophages fully induced the differentiation of adipocytes.Catechin has an anti-inflammatory effect by regulating the gene expression of cytokines via AMPK/SIRT1 signaling pathway.

关 键 词：儿茶素 脂肪细胞 细胞炎性因子 AMPK SIRT1 

分 类 号：TS272[农业科学—茶叶生产加工] TS201.4[轻工技术与工程—农产品加工及贮藏工程]