浓香型白酒发酵糟醅中乙酸乙酯含量测定方法的研究  

Determination Methods of Ethyl Acetate in Fermented Grains of Nongxiang Baijiu

作　　者：彭奎[1,2,3,5] 刘义会 张磊[1,2,3,4,5] 王超凯 李觅[1,2,3,4,5] 常少健 郭杰[1,2,3,5] 蔡海燕 孙中理 刘念[1,2,3,4,5] PENG Kui;LIU Yihui;ZHANG Lei;WANG Chaokai;LI Mi;CHANG Shaojian;GUO Jie;CAI Haiyan;SUN Zhongli;LIU Nian(Sichuan Food Fermentation Industry Research&Design Institute,Chengdu,Sichuan 611130;Key Laboratory of Brewing Engineering and Application of China National Light Industry Council,Chengdu,Sichuan 611130;Liu Nian's Master Studio of Liquor-making Skills,Chengdu,Sichuan 611130;Liquor Making Bio-technology&Application Key Laboratory,Yibin,Sichuan 644000;National Engineering Research Center of Solid-state Brewing,Luzhou,Sichuan 646000,China)

机构地区：[1]四川省食品发酵工业研究设计院,四川成都611130 [2]中国轻工业酿酒工程及应用重点实验室,四川成都611130 [3]刘念酿酒师技能大师工作室,四川成都611130 [4]酿酒生物技术及应用四川省重点实验室,四川宜宾644000 [5]国家固态酿造工程技术研究中心,四川泸州646000

出　　处：《酿酒科技》2021年第5期98-100,共3页Liquor-Making Science & Technology

基　　金：四川省科技厅重点研发项目(2019YFS0521);四川省科技厅重点研发项目(2019YFN0004);酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2019-07)。

摘　　要：发酵后期糟醅以及出窖糟醅,宜选用与乙醇溶液混合蒸馏,蒸馏时间30 min,蒸馏液进行气相色谱分析来测定糟醅中乙酸乙酯含量。此方法重现性好,回收率较高,操作简单,适用于日常测定。入窖糟醅及发酵前期糟醅宜选用与乙醇溶液混合、超声15 s,静置10 min,纱布过滤,滤液进行蒸馏,蒸馏时间30 min,蒸馏液进行气相色谱分析来测定糟醅中乙酸乙酯含量。此方法回收率较低但重复性好,较稳定,可以用于跟踪糟醅中乙酸乙酯变化情况研究等。In the late fermentation stage and after fermentation,the fermented grains was mixed with ethanol solution for distillation for 30 min,and the distillate was analyzed by gas chromatography to determine the content of ethyl acetate in the fermented grains.This method had good reproducibility,high recovery rate,and simple operation,and was suitable for daily determination.In the early stage of fermentation,the fermented grains was mixed with ethanol solution,went through ultrasonic treatment for 15 s,stood for 10 min,and filtered by gauze.The filtrate was distilled for 30 min,and the distillate was analyzed by gas chromatography to determine the content of ethyl acetate in the fermented grains.This method had low recovery rate,yet its repeatability and stability were good,and could be used to track the changes of ethyl acetate in the fermented grains.

关 键 词：糟醅 乙酸乙酯 蒸馏 色谱分析 含量测定 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]