基于全基因组序列的污染事件中椰毒假单胞菌酵米面亚种溯源分析  

Traceability analysis of Pseudomonas cocovenenans subsp.Farino fermentans in pollution events based on whole genome sequence

作　　者：陈荣桥 陈汉金 冼燕萍 侯向昶 朱文信 郭燕华 胡均鹏 梁明 刘冬虹 吴玉銮 CHEN Rong-Qiao;CHEN Han-Jin;XIAN Yan-Ping;HOU Xiang-Chang;ZHU Wen-Xin;GUO Yan-Hua;HU Jun-Peng;LIANG Ming;LIU Dong-Hong;WU Yu-Luan(Guangzhou Quality Supervision and Testing Institute,Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety,Guangzhou City Key Laboratory of Detection Technology for Food Safety,Guangzhou 511447,China)

机构地区：[1]广州质量监督检测研究院,广州市食品安全风险动态监测与预警研究中心,广州市食品安全检测技术重点实验室,广州511447

出　　处：《食品安全质量检测学报》2021年第9期3854-3859,共6页Journal of Food Safety and Quality

基　　金：国家市场监督管理总局研究项目;广东省科技计划项目(2019B020208008)。

摘　　要：目的溯源分析湿米粉与淀粉制品(统称为"湿粉")及其原料米中分离的椰毒假单胞菌酵米面亚种。方法采用GB/T 4789.29—2003《食品安全国家标准食品卫生微生物学检验椰毒假单胞菌酵米面亚种检验》在14份湿粉及其原料米中分离出34株唐菖蒲伯克霍尔德氏菌并进行菌株全基因组重测序,以BurkholderiagladioliCo14作为参比基因,基于单核苷酸多态性(single nucleotide polymorphism,SNP)数据构建进化树,分析不同菌株的同源关系。结果来源于相同产地标识的样品的菌株呈现较好聚类;来源于同一生产企业的湿粉和碎米样品的菌株具有高度同源关系。结论提示原料米中椰毒假单胞菌酵米面亚种的基因组序列与产地溯源具有较大相关性,在湿粉生产加工过程中存在椰毒假单胞菌酵米面亚种污染传递的风险。Objective To trace and analyze Pseudomonas cocovenenans subsp.Farino fermentans isolated from wet rice flour,starch products(collectively referred to as"wet rice flour")and raw rice.Methods A total of 34 strains of Burkholderia gladioli were isolated from 14 portions of wet flour and its raw rice and the whole genome of the strain was re-sequenced using GB/T 4789.29—2003 National food safety standard-Microbiological examination of food hygiene-Examination of Pseudomonas cocovenenans subsp.Farino fermentans.With Burkholderia gladioliCo14 as the reference gene,an evolutionary tree was constructed based on single nucleotide polymorphism data,and then the homology relationship of different strains were analyzed.Results The strains from the samples with the same origin identification showed better clustering.The strains of wet powder and broken rice samples from the same manufacturer had a high degree of homology.Conclusion It is suggested that the genome sequence of Pseudomonas cocovenenans subsp.Farino fermentans in raw rice is closely related to the origin traceability,and there is a risk of pollution transmission of Pseudomonas cocovenenans subsp.Farino fermentans in the production and processing of wet flour.

关 键 词：椰毒假单胞菌酵米面亚种 湿粉及其原料米 全基因组序列 进化树 溯源 

分 类 号：TS207.4[轻工技术与工程—食品科学]