离子色谱-脉冲安培法检测食品中功能性低聚异麦芽糖  

Determination of Isomaltooligosaccharide in Foods by Ion Chromatography-pulsed Amperometric Detection

作　　者：徐佳佳[1] 刘玉峰[1] 孔凡华 乔子纯 崔亚娟[1] 徐锡媛 郑晓玮 李东[1] XU Jiajia;LIU Yufeng;KONG Fanhua;QIAO Zichun;CUI Yajuan;XU Xiyuan;ZHENG Xiaowei;LI Dong(Beijing Institute of Nutrition Resources,Beijing 100069,China)

机构地区：[1]北京市营养源研究所,北京100069

出　　处：《食品工业科技》2021年第13期262-267,共6页Science and Technology of Food Industry

基　　金：十三五国家重点研发计划-营养功能性食品制造关键技术研究与新产品创制(2016YFD0400600)。

摘　　要：目的:建立一种离子色谱-脉冲安培检测法测定食品中的异麦芽糖、异麦芽三糖、潘糖的方法。方法:样品经水溶解提取,酸沉淀蛋白后,过反相固相萃取柱净化,以赛默飞CarboPacTM PA20阴离子交换柱为分析柱,以水(A)、200 mmol/L氢氧化钠(B)为淋洗液梯度洗脱,离子色谱-脉冲安培检测器定量测定。结果:在0.8~16.0 mg/L范围内线性关系良好,相关系数r均大于0.999,异麦芽糖、异麦芽三糖、潘糖精密度RSD分别为3.42%、2.34%、1.11%,回收率在85.63%~105.22%之间,相对标准偏差在2.48%~4.49%之间。结论:该方法前处理简单,专一性强,结果准确度高,适用于多种食品中的低聚异麦芽糖的测定。Objective:An ion chromatography-pulsed amperometric detection method was developed for the determination of isomaltose,isomaltotriose,panose in foods.Methods:Samples were extracted by water and the protein in sample was deposited by acid,and purified with the column of guardⅡRP,then those sugars were separated on a CarboPac PA20 high performance anion-exchange column of ThermoFisher.The water(A),200 mmol/L sodium hydroxide solution(B)were used as mobile phases for gradient elution,and pulsed amperometric detector was used for quantitative determination.Results:The linear relationship was good within the range of 0.8~16.0 mg/L,the correlation coefficient was more than0.999,the RSD of precision of isomaltose、isomaltotriose and panose were 3.42%,2.34%,1.11%,the recovery rate was85.63%~105.22%,the RSD was 2.48%~4.49%.Conclusion:This method demonstrated to be simple with good accuracy and specifity,which is suitable for the determination of isomaltooligosaccharide in kinds of foods.

关 键 词：异麦芽糖 异麦芽三糖 潘糖 离子色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]