食源性混合病原菌生物被膜行为对金黄色葡萄球菌毒素因子转录水平的影响  

Effect of Food-borne Mixed Bacteria Biofilm Behavior on the Transcriptional Level of Virulence Genes of S.aureus

作　　者：陈忠军[1] 张钰皎 胡炜东[2] 孙子羽 苏杰[2] 满都拉[1] Chen Zhongjun;Zhang Yujiao;Hu Weidong;Sun Ziyu;Su Jie;Mandlaa(College of Food Science and Engineering,Inner Mongolia Agricultural University,Huhhot 010018;Vocational and Technical College,Inner Mongolia Agricultural University,Baotou 014109,Inner Mongolia)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特010018 [2]内蒙古农业大学职业技术学院,内蒙古包头014109

出　　处：《中国食品学报》2021年第6期287-295,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：内蒙古草原英才项目(DC2000000742);内蒙古自治区高等学校科学研究项目(NJZZ17053)。

摘　　要：以食源性病原菌(金黄色葡萄球菌、大肠杆菌和肠炎沙门氏菌)形成的混合菌生物被膜为研究对象,对其定性和定量检测,通过实时荧光定量PCR方法分别检测单一菌和混合菌生物被膜中金黄色葡萄球菌28种目的毒素基因转录水平的变化。结果表明,结晶紫染色法、银染法和扫描电镜法均表明混合菌在培养24 h时达到最大黏附度;混合菌生物被膜中3种细菌的活菌数均低于单菌生物被膜中的活菌数;28种毒素基因在金黄色葡萄球菌中均被检出,且混合菌相对于单一菌生物被膜,上调量显著的有肠毒素基因sep、ser,显著下降的有溶血素基因hla和脱皮毒素基因etd,肠毒素基因seb表达量相等。In the research,a mixed bacterial biofilm formed by foodborne pathogens(Staphylococcus aureus,Escherichia coli and Salmonella enterica)was used as a research object.After qualitative and quantitative detection,Real Time PCR method was used to detect single and mixed bacterial biofilms.Changes in the transcriptional levels of 28 toxin genes of Staphylococcus aureus was studied by Real Time PCR method.The results showed that the crystal violet staining method,silver staining method and scanning electron microscopy method all proved that the mixed bacteria reached the maximum adhesion when cultured for 24 h;the number of viable bacteria of the three bacteria in the mixed biofilm was lower than that of the single bacteria.The number of the toxins,28 toxin genes were detected in Staphylococcus aureus;mixed bacteria compared to single bacterial biofilm,the up-regulation of the enterotoxin gene sep,ser,significantly decreased hemolysin hla and ecdysone etd The enterotoxin seb is expressed in equal amounts.

关 键 词：混合菌生物被膜 荧光定量PCR 银染法 扫描电镜 毒素因子 

分 类 号：TS201.3[轻工技术与工程—食品科学]