(+)-儿茶素与表没食子儿茶素没食子酸酯对乙醇诱导HepG2细胞脂代谢紊乱及氧化应激的影响  

Effects of(+)-Catechin and Epigallocatechin Gallate on Ethanol-Induced Lipid Accumulation and Oxidative Stress in HepG2 Cells

作　　者：胡博然[1] 丁建才 曹杨 田颖[1] 国凤华 袁静[2] HU Boran;DING Jiancai;CAO Yang;TIAN Ying;GUO Fenghua;YUAN Jing(School of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China;Capital Institute of Pediatrics,Beijing 100020,China;Tonghua Winery Co.,Ltd.,Tonghua 134002,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225127 [2]首都儿科研究所,北京100020 [3]通化葡萄酒股份有限公司,吉林通化134002

出　　处：《食品科学》2021年第13期114-120,共7页Food Science

基　　金：国家自然科学基金面上项目(31370093)。

摘　　要：目的:体外对比(+)-儿茶素((+)-catechin,(+)-Cat)与表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对乙醇诱导的HepG2细胞脂代谢紊乱及氧化应激的差异。方法:以HepG2细胞为实验对象,乙醇作用组(ETOH组)加入作用浓度300 mmol/L乙醇,(+)-Cat+ETOH组加入200μmol/L(+)-Cat和300 mmol/L乙醇,EGCG+ETOH组加入200μmol/L EGCG和300 mmol/L乙醇;另设相同浓度的(+)-Cat组、EGCG组及正常组,各组均在37℃培养24 h。检测各组HepG2细胞甘油三酯(triglyceride,TG)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛(malondialdehyde,MDA)浓度;油红O染色观察各组HepG2细胞的脂滴状态;荧光定量聚合酶链式反应法检测各组HepG2细胞固醇调节元件结合蛋白1(sterol regulatory element binding protein 1,SREBP-1)、二脂酰甘油转移酶2(diacylglyceryltransferase 2,DGAT2)、过氧化物酶体增殖物激活受体α(peroxisomal proliferators activate receptorsα,PPARα)、肉毒碱棕榈酰基转移酶1(carnityltransferase 1,CPT1)mRNA相对表达水平。结果:ETOH组细胞发生氧化应激,同时TG含量明显高于正常组、(+)-Cat组和EGCG组;(+)-Cat+ETOH组和EGCG+ETOH组细胞氧化应激反应得到明显改善,同时TG含量显著低于ETOH组(P<0.05,P<0.01),并且显著下调SREBP-1和DGAT2 mRNA表达量(P<0.05,P<0.01),上调PPARα和CPT1 mRNA表达量(P<0.05,P<0.01)。结论:(+)-Cat与EGCG均能改善乙醇诱导的HepG2细胞氧化应激反应和脂代谢紊乱,且EGCG的效果更好。Objective:To compare in vitro effects of(+)-catechin(Cat)and epigallocatechin gallate(EGCG)on ethanol(ETOH)-induced aberrant lipid metabolism and oxidative stress in HepG2 cells.Methods:HepG2 cells were divided into six groups:normal,200μmol/L(+)-Cat,200μmol/L EGCG,300 mmol/L ETOH treatment,200μmol/L(+)-Cat plus 300 mmol/L ETOH treatment,and 200μmol/L EGCG plus 300 mmol/L ETOH treatment.All groups were cultured at 37℃for 24 h.Thereafter,the contents of triglyceride and malondialdehyde(MDA)and superoxide dismutase(SOD)activity were determined.The morphology of lipid droplets in HepG2 cells in each group was observed by Oil Red O staining.The mRNA expression of sterol regulatory element binding protein 1(SREBP-1),peroxisomal proliferators activate receptorsα(PPARα),carnityltransferase 1(CPT1)and diacylglyceryltransferase 2(DGAT2)in HepG2 cells were measured by fluorescence quantitative polymerase chain reaction.Results:The level of oxidative stress and TG content in ETOH-treated cells were significantly higher than those in the normal,(+)-Cat and EGCG groups.The oxidative stress response in the(+)-Cat plus ETOH and EGCG plus ETOH groups was significantly improved,and TG content was significantly lower in the two groups than in the ETOH group(P<0.05,P<0.01).Moreover,the mRNA expression of SREBP-1 and DGAT2 was down-regulated(P<0.05,P<0.01),and the mRNA expression of PPARαand CPT1 was up-regulated(P<0.05,P<0.01).Conclusion:Both(+)-Cat and EGCG can improve ETOH-induced oxidative stress and lipid metabolism disorders in HepG2 cells,and EGCG is more effective.

关 键 词：(+)-儿茶素 表没食子儿茶素没食子酸酯 脂代谢 氧化应激 

分 类 号：R151.2[医药卫生—营养与食品卫生学]