调味香料荜茇精油中清除游离基活性成分的快速筛选  

Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L.as Food Seasoning

作　　者：王婵 李荣[1] 吴杨 王颖[1] 汤书华 谭津 姜子涛[1,3] WANG Chan;LI Rong;WU Yang;WANG Ying;TANG Shuhua;TAN Jin;JIANG Zitao(School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin 300134,China;Tianjin Evans Science and Technology Co.Ltd.,Tianjin 300384,China;School of Food Engineering,Tianjin Tianshi College,Tianjin 301700,China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津埃文森科技有限公司,天津300384 [3]天津天狮学院食品工程学院,天津301700

出　　处：《食品科学》2021年第14期226-231,共6页Food Science

基　　金：天津市自然科学基金项目(16JCYBJC43300,17JCQNJC02400)。

摘　　要：利用超快速气相色谱电子鼻(gas chromatography-based electronic nose,GC-E-nose)技术快速鉴定调味香料荜茇精油(essential oil from Piper longum L.,EOPL)的化学成分,并通过GC-E-nose结合化学法,在未经单体分离的条件下,对EOPL中的化学成分清除1,1-二苯基-2-三硝基苯肼(1,1-dipheny1-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)阳离子自由基和羟自由基活性进行评价。结果表明,EOPL的主要成分为大根香叶烯(26.04%)、石竹烯(13.57%)、顺式-茴香脑(8.87%)和β-甜没药烯(7.32%)。EOPL各组分清除自由基的活性有很大差异,在所有精油成分中顺式-茴香脑、氧化石竹烯和右旋萜二烯对DPPH自由基、ABTS阳离子自由基和羟自由基具有最强的清除活性,清除率分别达到78.1%、87.0%和71.6%。为了验证GC-E-nose对精油成分鉴定的可行性,同时采用气相色谱-质谱联用技术对EOPL的化学成分进行分析,符合度高达89.7%。In the present work,the chemical components of the essential oil from Piper longum L.(EOPL)were quickly identified with an ultra-fast gas chromatography-based electronic nose(GC-E-nose).The scavenging activities of each component of EOPL against 1,1-dipheny1-2-picrylhydrazyl(DPPH)radicals,2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)cation radicals,and hydroxyl radicals were evaluated by GC-E-nose combined with chemical methods without time-consuming and laborious separation of the monomers.The results showed that the main components of EOPL identified by GC-E-nose were germacrene D(26.04%),caryophyllene(13.57%),cis-anetholel(8.87%),andβ-bisabolene(7.32%).Among all identified components,cis-anethol,caryophyllene oxide,and D-limonene had the strongest scavenging capacity against DPPH radicals,ABTS cation radicals,and hydroxyl radicals,with scavenging percentages of 78.1%,87.0%,and 71.6%,respectively.The results of GC-E-nose for essential oil components were 89.7%consisted with those of gas chromatography-mass chromatography(GC-MS),confirming the feasibility of GC-E-nose.

关 键 词：荜茇精油 超快速气相色谱电子鼻 气相色谱-质谱联用技术 快速筛选 清除自由基 

分 类 号：TS201.3[轻工技术与工程—食品科学]