四川太和毛霉豆豉中优势发酵菌株的分离鉴定与酶活分析  

Isolation, Purification Enzyme Activity Analysis of Domianant Fermentation Strains from Sichuan Taihe Mucor Douchi

作　　者：何维 安天星 余玲 廖柯 陈超 刘迎涛 胡洁 邓婷 尹鑫 苏波 赵志峰[2] HE Wei;AN Tian-xing;YU Ling(College of Biomass Science and Engineering,Sichuan University,Chengdu,Sichuan 610065)

机构地区：[1]成都太和坊酿造有限公司,四川成都610501 [2]四川大学轻工科学与工程学院,四川成都610651

出　　处：《安徽农业科学》2021年第14期157-161,共5页Journal of Anhui Agricultural Sciences

基　　金：四川省省级财政科技计划重点研发项目(2018GZ0022)。

摘　　要：[目的]从传统的四川太和毛霉豆豉中分离产蛋白酶较高的优势菌株,并通过形态学与分子生物学技术进行鉴定,研究菌株酶活。[方法]以四川太和毛霉豆豉为研究对象,通过分离、纯化技术分离其中优势菌,并结合形态学与分子生物学技术进行特征菌落鉴定,最后对2株霉菌的蛋白酶和纤维素酶活性进行检测。[结果]四川太和毛霉豆豉中2株产蛋白酶较高的优势菌株分别为M-THF-01与M-THF-02;经形态学与分子生物学鉴定技术发现,M-THF-01为雅致放射毛霉(Actinomucor elegans),M-THF-02为总状毛霉(Mucor racemosa)。M-THF-01与M-THF-02中性蛋白酶酶活分别为(83.00±4.25)U/mL和(277.35±6.09)U/mL,酸性蛋白酶分别为(156.36±3.68)U/mL和(405.69±4.18)U/mL,碱性蛋白酶分别为(19.30±2.56)U/mL和(25.37±3.88)U/mL,产纤维素总体酶活分别为(2.51±0.85)U/mL和(3.64±1.31)U/mL。总体来说,M-THF-02产酶活力明显高于M-THF-01。[结论]该研究可为毛霉豆豉纯复合发酵剂的研发提供理论基础,并为毛霉豆豉的工业化与规模化生产提供科学依据。[Objective]To isolate the dominant strain with high protease production from the traditional Sichuan Taihe Mucor Douchi,and identify the strains by morphology and molecular biology technology and study the enzyme activity of the strains.[Method]In this study,Mucor Taihe Douchi was used as the research object.The dominant bacteria were isolated by separation and purification technology,and the characteristic colonies were identified by morphological and molecular biological techniques.Finally,the protease and cellulase activities of the two strains were detected.[Result]The results showed that M-THF-01 and M-THF-02 were the two dominant protease producing strains in Sichuan Taihe Mucor Douchi,and M-THF-01 was Actinomucor elegans and THF-02 was Mucor racemosus.The neutral protease activities of M-THF-01 and M-THF-02 were(83.0±4.25)U/mL and(277.35±6.09)U/mL,respectively;acid protease were(156.36±3.68)U/mL and(405.69±4.18)U/mL;alkaline protease were(19.3±2.56)U/mL and(25.37±3.88)U/mL;the total cellulase activity of M-THF-01 and M-THF-02 were(2.51±0.85)U/mL and(3.64±1.31)U/mL respectively;in general,the enzyme activity of M-THF-02 was significantly higher than that of M-THF-01.This study provides a theoretical basis for the development of Mucor Douchi pure compound starter,and provides the scientific basis for the industrialization and large-scale production of Mucor Douchi.

关 键 词：豆豉 分离 鉴定 雅致放射毛霉 总状毛霉 酶活 

分 类 号：TS201.3[轻工技术与工程—食品科学]