Pramipexole, a dopamine D3/D2 receptor-preferring agonist, attenuates reserpine-induced fibromyalgia-like model in mice  

作　　者：Carlos Pereira Martins Rodrigo Sebben Paes Gabriela Mantovani Baldasso Eduarda Gomes Ferrarini Rahisa Scussel Rubya Pereira Zaccaron Ricardo Andrez Machado-de-Avila Paulo Cesar Lock Silveira Rafael Cypriano Dutra 

机构地区：[1]Laboratory of Autoimmunity and Immunopharmacology,Department of Health Sciences,Campus Ararangua,Universidade Federal de Santa Catarina,Araranguá,SC,Brazil [2]Post-Graduate Program of Neuroscience,Center of Biological Sciences,Universidade Federal de Santa Catarina,Florianópolis,SC,Brazil [3]Laboratory of Experimental Physiopathology,Program of Postgraduate in Science of Health,Universidade do Extremo Sul Catarinense,Criciuma,SC,Brazil

出　　处：《Neural Regeneration Research》2022年第2期450-458,共9页中国神经再生研究（英文版）

基　　金：supported by grants from Programa de Pos-graduacao em Neurociencias(PGN),Programa INCT-INOVAMED(grant No.465430/2014-7);Fundacao de Apoio à Pesquisa e Inovacao do Estado de Santa Catarina(FAPESC);Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)。

摘　　要：Fibromyalgia(FM) is a complex pathology described as persistent hyperalgesia including somatic and mood dysfunctions, depression and anxiety. Although the etiology of FM is still unknown, a significant decrease in biogenic amines is a common characteristic in its pathogenesis. Here, our main objective was to investigate the role of dopamine D3/D2 receptor during the reserpine-induced pain in mice. Our results showed that pramipexole(PPX) – a dopaminergic D3/D2 receptor agonist – inhibited mechanical allodynia and thermal sensitivity induced by reserpine. Relevantly, PPX treatment decreased immobility time and increased the number of grooming in the forced swimming test and splash test, respectively. Animals that received PPX remained longer in the open arms than the reserpine group using elevated plusmaze apparatus. The repeated PPX administration, given daily for 4 days, significantly blocked the mechanical and thermal allodynia during FM model, similarly to pregabalin, although it failed to affect the reserpine-induced thermal nociception. Reserpine administration induced significant downregulation of dopamine concentration in the central nervous system, and repeated treatment with PPX restored dopamine levels in the frontal cortex and spinal cord tissues. Moreover, PPX treatment inhibited oxidants production such as DCFH(2′,7′-dichlorodihydrofluorescein) and nitrite, also decreased oxidative damage(carbonyl), and upregulated the activity of superoxide dismutase in the spinal cord. Together, our findings demonstrated the ability of dopamine D3/D2 receptor-preferring agonist in reducing pain and mood dysfunction allied to FM in mice. All experimental protocols were approved by the Universidade Federal de Santa Catarina(UFSC) Ethics Committee(approval No. 2572210218) on May 10, 2018.

关 键 词：DOPAMINE dopaminergic system FIBROMYALGIA HYPERALGESIA pain PRAMIPEXOLE RESERPINE 

分 类 号：R965[医药卫生—药理学]