南极磷虾油改善虾青素生物利用度的研究  

Studies on the Improvement of Astaxanthin Bioavailability by Antarctic Krill Oil

作　　者：刘小芳[1] 刘建志 唐一新 孙伟红[1] 张辉珍 冷凯良[1,4] Liu Xiaofang;Liu Jianzhi;Tang Yixin;Sun Weihong;Zhang Huizhen;Leng Kailiang(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Sustainable Development of Polar Fishery,Ministry of Agriculture and Rural Affairs,Qingdao 266071,Shandong;School of Public Health,Qingdao University,Qingdao 266021,Shandong;Qingdao Institute for Food and Drug Control,Qingdao 266071,Shandong;Laboratory for Marine Drugs and Bioproducts,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266200,Shandong)

机构地区：[1]中国水产科学研究院黄海水产研究所,农业农村部极地渔业开发重点实验室,山东青岛266071 [2]青岛大学医学部公共卫生学院,山东青岛266021 [3]青岛市食品药品检验研究院,山东青岛266071 [4]青岛海洋科学与技术试点国家实验室,海洋药物与生物制品功能实验室,山东青岛266200

出　　处：《中国食品学报》2021年第7期141-148,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC1406806)。

摘　　要：研究南极磷虾油对天然虾青素生物利用度的影响。将雄性Wistar大鼠随机分为空白对照组、虾青素组、南极磷虾油+虾青素组、鱼油+虾青素组,按照成人每日摄入南极磷虾油2 g/d或鱼油2 g/d、虾青素12 mg/d等量换算,灌胃给予大鼠脂肪乳剂,空白对照组给予等量猪胆盐溶液,检测灌胃后不同时间节点大鼠肠道内容物、小肠绒毛、血清中虾青素含量的变化,根据血清AUC0-t值评价生物利用度。结果显示,南极磷虾油+虾青素组和鱼油+虾青素组大鼠肠道内容物、小肠绒毛、血清中虾青素检出量较虾青素组显著提高(P<0.05)。南极磷虾油+虾青素组大鼠血清虾青素含量峰值为(15.8±2.09)μg/L,是鱼油+虾青素组【(3.94±0.27)μg/L】的4.02倍(P<0.05),是虾青素组【(1.89±0.26)μg/L】的8.37倍(P<0.05)。南极磷虾油+虾青素组大鼠血清AUC0-t值为(108.4±2.34)μg·h/L,是鱼油+虾青素组【(23.4±0.46)μg·h/L】的4.63倍(P<0.05),是虾青素组【(14.4±0.21)μg·h/L】的7.55倍(P<0.05)。南极磷虾油可显著改善虾青素的生物利用度,效果优于鱼油。本研究可为新型南极磷虾油衍生产品的开发提供理论依据。In the present study,the effect of Antarctic krill oil on the bioavailability of the natural astaxanthin extract from green alga(Haematococcus pluvialis)was evaluated using male Wistar rats as model animals,which were randomly divided into the control group,astaxanthin group,krill oil+astaxanthin group and fish oil+astaxanthin group.The experimental group rats were intragastrically administered with the corresponding fat emulsion while the control group rats were given the bile salts solution.The gavage doses were converted according to the daily intake of 2 g krill oil or 2 g fish oil per day and 12 mg astaxanthin per day for adults.The astaxanthin levels in intestinal contents,intestinal villus and serum of rats at different time points after gavage were detected and the bioavailability was evaluated using the serum AUC0-t value.The results showed that the astaxanthin levels in intestinal contents,intestinal villus and serum of rats from krill oil+astaxanthin group and fish oil+astaxanthin group were significantly higher when compared with the astaxanthin group(P<0.05).The peak value of the astaxanthin level in the serum of rats from krill oil+astaxanthin group was(15.8±2.09)μg/L,which was 4.02 times(P<0.05)of that in the fish oil+astaxanthin group[(3.94±0.27)μg/L],and 8.37 times(P<0.05)of that in the astaxanthin group[(1.89±0.26)μg/L].More importantly,the AUC0-t value of rats from krill oil+astaxanthin group was(108.4±2.34)μg·h/L,which was 4.63 times(P<0.05)of that in the fish oil+astaxanthin group[(23.4±0.46)μg·h/L],and 7.55 times(P<0.05)of that in the astaxanthin group[(14.4±0.21)μgo·/L].Antarctic krill oil could significantly ameliorate the bioavailability of the natural astaxanthin in rats and the effect is better than that of fish oil.This study would provide scientific guidance and theoretical support for the development of new Antarctic krill oil derivatives.

关 键 词：南极磷虾油 虾青素 生物利用度 鱼油 大鼠 

分 类 号：TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程]