反胶束法提取辣木籽蛋白工艺优化  

Optimization of extraction process of Moringa oleifera seed protein by reverse micelle method

作　　者：和丽 吴君瑞 王雪峰[1,2] 普岳红[1,2] 范江平[1,2] 王桂瑛[1,2] 肖智超 徐志强[1,2] 谷大海[1,2] 黄艾祥[1] HE Li;WU Junrui;WANG Xuefeng;PU Yuehong;FAN Jiangping;WANG Guiying;XIAO Zhichao;XU Zhiqiang;GU Dahai;HUANG Aixiang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Engineering Technology Research Center for Processing of Livestock Products,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201 [2]云南农业大学畜产品加工工程技术研究中心,昆明650201

出　　处：《中国油脂》2021年第7期80-85,98,共7页China Oils and Fats

基　　金：国家自然科学基金地区项目(31960462);云南省基础研究计划面上项目(2019FB052);云南省农业联合专项(2018FG001-040)。

摘　　要：以脱脂辣木籽粉为原料,利用反胶束法提取辣木籽蛋白并进行工艺优化。通过单因素实验和正交实验考察反胶束(AOT)质量浓度、提取温度、pH、料液比、水分活度(Wo)5个因素对提取辣木籽蛋白的反胶束前萃工艺的影响,再通过单因素实验和二次通用旋转实验考察提取温度、pH、KCl浓度、提取时间4个因素对提取辣木籽蛋白的超声波辅助反胶束后萃工艺的影响。结果表明:反胶束前萃最佳工艺条件为pH 9、料液比1∶50、AOT质量浓度0.08 g/mL、提取温度45℃、水分活度25,该条件下所得前萃辣木籽蛋白提取率为67.2%;超声波辅助反胶束后萃最佳工艺条件为提取时间45 min、提取温度45℃、pH 6.5、KCl浓度1.25 mol/L,在此条件下所得后萃辣木籽蛋白提取率为58.5%。Using defatted Moringa oleifera seed powder as raw material, the reverse micelle method was used to extract Moringa oleifera seed protein and the process was optimized. Through single factor experiment and orthogonal experiment, the effects of the reverse micelle(AOT)mass concentration, extraction temperature, pH, ratio of material to liquid, and water activity on the reverse micelle pre-extraction process for extracting Moringa oleifera seed protein were investigated. The single factor experiment and the second general rotating experiment were used to investigate the ultrasound-assisted reverse micelle post-extraction process of extracting Moringa oleifera seed protein with four factors of extraction temperature, pH, KCl concentration, and extraction time. The results showed that the optimal process conditions for the reverse micelle pre-extraction were obtained as follows: pH 9, ratio of material to liquid 1∶50, AOT mass concentration 0.08 g/mL, extraction temperature 45℃, water activity 25. Under these conditions, the extraction rate of pre-extracted Moringa oleifera seed protein was 67.2%. The optimal process conditions for ultrasound-assisted reverse micelle post-extraction were obtained as follows: extraction time 45 min, extraction temperature 45℃, pH 6.5, and KCl concentration 1.25 mol/L. Under these conditions, the extraction rate of post-extracted Moringa oleifera seed protein was 58.5%.

关 键 词：辣木籽 辣木籽蛋白 反胶束 前萃 后萃 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ936.2[轻工技术与工程—食品科学与工程]