食源性致病微生物检测技术研究进展  

Research progress of detection technologies for foodborne pathogens

作　　者：钱佳婕 黄迪 徐颖华[2] 徐志南[1] QIAN Jia-Jie;HUANG Di;XU Ying-Hua;XU Zhi-Nan(College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China;National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]浙江大学化学工程与生物工程学院,杭州310027 [2]中国食品药品检定研究院,北京102629

出　　处：《食品安全质量检测学报》2021年第12期4775-4785,共11页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2018YFC1603902);杭州市科技发展计划项目(20202013A02)。

摘　　要：食源性致病微生物是引起食品安全问题的重要因素。针对食源性致病微生物,快速而准确的检测是保障食品安全的关键举措。随着微生物学、分子生物学技术的发展,基于新型基因组编辑技术和先进生物传感器的检测技术不断涌现,食源性致病微生物的检测技术展现出多样性和综合性的特点,并得到了广泛应用和商业化发展。本文从食源性致病微生物分类出发,深入总结和探讨传统培养分离法、免疫学检测技术、核酸检测技术和生物传感器检测技术的优缺点,并重点介绍了近年来开发的基于核酸等温扩增技术和CRISPR基因编辑技术的核酸检测新方法。通过对最新的针对食源性致病微生物检测方法进行综述,为研究者开辟食源性致病微生物检测新方法提供重要的理论参考。Foodborne pathogens are an important cause of many food safety problems. Rapid and accurate detection of foodborne pathogens is a key measure to ensure food safety. With the development of microbiology and molecular biology technology, detection technologies based on novel genome editing technology and advanced biosensor continues to emerge. Pathogenic microorganism detection technologies have shown the characteristics of diversity and comprehensiveness, which have been widely applied and commercialized. Based on the classification of foodborne pathogens, this review thoroughly summarized and analyzed the advantages and disadvantages in allusion to culture-based methods, immunological detection technologies, nucleic acid detection technologies and biosensor detection technologies, with emphasis on the introduction of emerging nucleic acid detection methods based on nucleic acid isothermal amplification technology and clustered regularly interspaced short palindromic repeats(CRISPR) gene editing technology developed in recent years. The review of the latest detection methods for foodborne pathogens provided an important theoretical reference for researchers to develop new detection methods for foodborne pathogens.

关 键 词：食源性致病微生物 核酸检测技术 CRISPR基因编辑技术 生物传感器检测技术 

分 类 号：TS207.4[轻工技术与工程—食品科学]