Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery  

作　　者：Yi Shen Ming-Jiang Yao Yu-Xin Su Dong-Sheng Xu Jia Wang Guang-Rui Wang Jing-Jing Cui Jian-Liang Zhang Wan-Zhu Bai 

机构地区：[1]Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing,China [2]Institute of Basic Medical Sciences,Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing,China [3]Beijing Key Laboratory of Pharmacology of Chinese Materia Medica,Beijing,China

出　　处：《Neural Regeneration Research》2022年第4期832-837,共6页中国神经再生研究（英文版）

基　　金：supported by the Project of National Key R&D Program of China,No.2019YFC1709103(to WZB);the National Natural Science Foundation of China,Nos.81774211(to WZB),81873040(to MJY),81774432(to JJC),81801561(to DSX),82004492(to JW)。

摘　　要：The mouse model of multiple cerebral infarctions,established by injecting fluorescent microspheres into the common carotid artery,is a recent development in animal models of cerebral ischemia.To investigate its effectiveness,mouse models of cerebral infarction were created by injecting fluorescent microspheres,45–53μm in diameter,into the common carotid artery.Six hours after modeling,fluorescent microspheres were observed directly through a fluorescence stereomicroscope,both on the brain surface and in brain sections.Changes in blood vessels,neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry.The microspheres were distributed mainly in the cerebral cortex,striatum and hippocampus ipsilateral to the side of injection.Microinfarcts were found in the brain regions where the fluorescent microspheres were present.Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia.These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts.This model is an effective,additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions.This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences(approval No.D2021-03-16-1)on March 16,2021.

关 键 词：ASTROCYTES blood-brain barrier common carotid artery fluorescent microsphere HISTOCHEMISTRY ISCHEMIA MICROGLIA microinfarcts NEURON neurovascular unit STROKE 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]