UVC-GA抑菌液对罗非鱼皮在0℃贮藏下微生物区系及品质的影响  

Effects of UVC-GA on the Microflora and Quality of Tilapia Skin Stored at 0℃

作　　者：吕敏 甘晖 何金钊 冯鹏霏 付玉春 陈秀荔 赵永贞 覃良举 马华威 LYU Min;GAN Hui;HE Jin-zhao;FENG Peng-fei;FU Yu-chun;CHEN Xiu-li;ZHAO Yong-zhen;QIN Liang-ju;MA Hua-wei(Guangxi Academy of Fishery Sciences,Nanning 530036,China;Guangxi Aquatic Animal and Husbandry College Aquaculture,Nanning 530035,China;Guangxi Aquaculture Beeding Center,Nanning 530031,China;Guangxi Agricultural Vocational and Technical College Food Engineering,Nanning 530073,China;Guangxi Xianyi Investment Co.Ltd.,Nanning 530044,China)

机构地区：[1]广西壮族自治区水产科学研究院,广西南宁530036 [2]广西水产畜牧学校水产系,广西南宁530035 [3]广西壮族自治区水产引育种中心,广西南宁530031 [4]广西农业职业技术学院食品工程系,广西南宁530073 [5]广西鲜之益投资有限公司,广西南宁530044

出　　处：《现代食品科技》2021年第8期142-151,共10页Modern Food Science and Technology

基　　金：广西重点研发计划项目(桂科AB19245013);广西重点实验室自主研究课题(19-A-02-04)。

摘　　要：紫外短波(UV-C)辐照没食子酸(GA)溶液12 h形成一种新的抑菌液(UVC-GA),与GA和去离子无菌水(DI)分别处理鱼皮后在0℃贮藏,DI组、GA组和UVC-GA组的贮藏期感官评价货架期分别为5 d、7 d和8 d。对优势菌属测定结果发现,1D组5 d(以下简称DI5)的优势菌属是Pseudoalteromonas(31.31%)、Candidatus-Bacilloplama(21.50%)和Litorimicrobium(17.11%),GA组7 d(以下简称GA7)的优势菌是Aliivibrio(42.40%)、Pseudoalteromonas(20.51%)和Psychrobater(10.32%),UVC-GA组8 d(以下简称UVC-GA8)的优势菌属Aliivibrio(29.81%)、C.Bacilloplama(20.90%)和Moritella(11.11%),UVC-GA8的Vibrio和Pseudoalteromonas相对含量均较低。对鱼皮品质相关指标检测发现,UVC-GA组的TVB-N、TVC、腐胺、尸胺、次黄嘌呤核苷(HxR)和次黄嘌呤(Hx)的含量均比较DI组和GA组低,但5′-单磷酸肌苷(IMP)含量高于其它组;UVC-GA8的K值显著低于DI5和GA7,随着贮藏时间延长UVC-GA组的pH值呈下降线性趋势,而DI组和GA组则呈“V”形。以上结果表明,UVC-GA组影响了罗非鱼皮在0℃贮藏下的微生物区系、TVB-N、TVC、生物胺、ATP复合物、K值等变化,应用UVC-GA抑菌液保鲜使得鱼皮货架期变长。A new antibacterial solution,UVC-GA was prepared by exposing gallic acid(GA)to UV-C for 12 h.Tilapia skins were treated with UVC-GA,GA,or deionized water(DI),and then stored at 0℃.Results showed that the sensory shelf-lives of the skin samples treated with DI,GA,and UVC-GA were five,seven,and eight days,respectively.The dominant bacterial genera in the DI-treated skins on day five(DI5)were Pseudoalteromonas(31.31%),Candidatus-Bacilloplama(21.5%),and Litorimicrobium(17.11%).The dominant genera in the GA-treated skins on day seven(GA7)were Aliivibrio(42.4%),Pseudoalteromonas(20.51%),and Psychrobacter(10.32%).The dominant bacterial genera in the UVC-GA-treated skins on day eight(UVC-GA8)were Aliivibrio(29.81%),Candidatus-Bacilloplama(20.9%)and Moritella(11.11%).The relative abundance of Aliivibrio and Pseudoalteromonas in UVC-GA8 was lower than that in DI5 and GA7.In addition,the amounts of quality-related chemical indicators,including TVB-N,TVC,putrescine,cadaverine,inosine,and hypoxanthine were lower in the UVC-GA-treated samples than those in the DI-and GA-treated samples;whereas inosine-5′-monophosphate content was higher in the UVC-GA-treated skins.The K value of UVC-GA8 was significantly lower than that of DI5 and GA7.V-shaped pH-time curves were obtained for both the DI and GA groups,whereas the pH decreased with time in the UVC-GA group.These results suggested that UVC-GA inhibited the deterioration of tilapia skin quality.The prolongation of the shelf life of tilapia skins might be attributed to UVC-GA-induced alterations in the microflora,changes in the contents of TVB-N,TVC,biogenic amines,and ATP complexes,and decrease in the K value of the skins stored at 0℃.

关 键 词：罗非鱼皮 没食子酸 紫外 微生物区系 品质 生物胺 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]