基于酪蛋白多极性pH变换的EGCG纳米粒制备及其稳定性研究  

Preparation of EGCG Nanoparticles Based on Casein Multipolar pH Transformation and Its Stability

作　　者：左小博 孔俊豪[1,2] 杨秀芳 房升[3] 彭新玲 谭蓉[1,2] 苏小琴 刁春华[1,2] Zuo Xiaobo;Kong Junhao;Yang Xiufang;Fang Sheng;Peng Xinling;Tan Rong;Su Xiaoqin;Diao Chunhua(Hangzhou Tea Research Institute,China COOP,Hangzhou 310016;Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resourses,Hangzhou 310016;School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018)

机构地区：[1]中华全国供销合作总社杭州茶叶研究院,杭州310016 [2]浙江省茶资源跨界应用技术重点实验室,杭州310016 [3]浙江工商大学食品与生物工程学院,杭州310018

出　　处：《中国食品学报》2021年第8期57-66,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2016YFD0400805,2018YFF0214203)。

摘　　要：针对茶多酚组分稳定性差,体系相容性不佳等限制其应用的问题,以表没食子儿茶素没食子酸酯(EGCG)为研究对象,通过多极性pH变换结合超声同步热诱导对酪蛋白(CS)改性,提高其包封载运能力。结合涡旋孵育建立自组装模型构建EGCG三元纳米粒体系,获得高荷载EGCG纳米粒体系,其包封率达90%~95%,装载率均高于305μg/mg,实现了多酚负载量显著提高(P<0.05)。在保证EGCG纳米粒较高装载率的同时,增强了体系稳定性和贮存稳定性,并具有较好的热力学性能和体外缓释特性,有利于推动EGCG高值化利用及应用领域扩展延伸。Disadvantages such as poor stability and compatibility,limit the health efficacy exertion and application of tea polyphenols.In this paper,casein(CS)was modified by multipolar pH transformation combined with ultrasonic simultaneous thermal induction to improve its stability of encapsulate and transport EGCG.The EGCG ternary nanoparticle emulsion was established by vortex incubation to establish a self-assembly model,and high-load EGCG nanoparticles were obtained.The encapsulation rate of the target product reached 90%-95%,and the loading rates were all higher than 305μg/mg,which achieved a significant increase in the loading of EGCG(P<0.05).In this study,the emulsion and storage stability of EGCG nanoparticles were enhanced while ensuring higher loading and encapsulation rates.Compared with the blank group,the thermodynamic stability and release properties in vitro of nano-encapsulated EGCG improved significantly,which was conducive to promoting the high-value utilization of EGCG and the expansion and extension of application fields.

关 键 词：EGCG纳米粒 酪蛋白 多极性pH变换 稳定性 缓释特性 

分 类 号：TS201.2[轻工技术与工程—食品科学]