高浓度蔗糖乙醇分批发酵动力学研究  

Study on Kinetics of Ethanol Batch Fermentation with High-concentration Sucrose

作　　者：刘明 伍时华[1,2] 龙秀锋[1,2] 易弋 曾令杰[1,2] LIU Ming;WU Shi-hua;LONG Xiu-feng;YI Yi;ZENG Ling-jie(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Key Laboratory of Green Processing of Sugar Resources in Guangxi Province,Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西科技大学广西糖资源绿色加工重点实验室,广西柳州545006

出　　处：《中国调味品》2021年第9期53-57,共5页China Condiment

基　　金：广西科技攻关项目(桂科攻0782003-2)。

摘　　要：以蔗糖为底物,按实验室酒精发酵工艺条件,接种酿酒酵母GJ2008于5 L发酵罐中发酵,每隔3 h取样分析酵母细胞数、乙醇浓度、总糖消耗情况,基于Logistic方程和Luedeking-Piret-like方程,利用Origin 9.5软件对发酵过程中酵母生长、乙醇生成、总糖消耗曲线进行动力学模拟,各自模型R 2分别为0.9929,0.9940,0.9975,绝大部分试验值和拟合值相对误差低于10%,表明模型能较好地反映和预测高浓度蔗糖乙醇分批发酵动态变化过程。In this study,sucrose is used as the substrate,according to the alcohol fermentation conditions in the laboratory,Saccharomyces cerevisiae GJ2008 is inoculated in a 5 L fermenter for fermentation,and the number of yeast cells,ethanol concentration and total sugar consumption are analyzed by sampling every 3 h.Based on Logistic equation and Luedeking-Piret-like equation,Origin 9.5 software is used to simulate the yeast growth,ethanol generation and total sugar consumption curves in the fermentation process,and the R 2 of each model is 0.9929,0.9940,0.9975 respectively.The relative errors of most experimental values and fitting values are less than 10%,which indicate that the model could well reflect and predict the dynamic process of ethanol batch fermentation of high-concentration sucrose.

关 键 词：蔗糖 乙醇 分批发酵 过程分析 动力学 

分 类 号：TS201.5[轻工技术与工程—食品科学]