气相色谱法测定椰子汁或复原椰子汁中5种香料  

Determination of 5 kinds of spices in coconut milk or reconstituted coconut milk by gas chromatography

作　　者：路露 何湘漪 孙蕊 耿健强 穆同娜 周相娟 LU Lu;HE Xiang-Yi;SUN Rui;GENG Jian-Qiang;MU Tong-Na;ZHOU Xiang-Juan(Beijing Food Safety Monitoring and Risk Assessment Center(Beijing Food Inspection Institute),Beijing 100094,China)

机构地区：[1]北京市食品安全监控和风险评估中心(北京市食品检验所),北京100094

出　　处：《食品安全质量检测学报》2021年第15期5990-5996,共7页Journal of Food Safety and Quality

基　　金：国家市场监督管理总局科技计划项目(2020MK008)。

摘　　要：目的建立气相色谱法检测椰子汁或复原椰子汁中乙基麦芽酚、香兰素、甲基香兰素、乙基香兰素和椰子醛的分析方法。方法取适量的椰子汁或复原椰子汁样品,使用硅藻土固相萃取柱净化提纯,使用乙酸乙酯作为洗脱剂洗脱,气相色谱法检测,外标法定量。结果乙基麦芽酚在5-100μg/mL范围内有较好的线性关系(r>0.990),检出限为2mg/kg,定量限为6mg/kg,回收率为88.4%-93.2%,相对标准偏差为2.8%-3.6%;香兰素、甲基香兰素和乙基香兰素在2-100μg/mL范围内有较好的线性关系(r>0.990),检出限为1 mg/kg,定量限为3 mg/kg,回收率为80.9%-114.4%,相对标准偏差为1.8%-5.9%;椰子醛在1-100μg/mL范围内有较好的线性关系(r>0.990),检出限为0.5mg/kg,定量限为1.5mg/kg,回收率为86.5%-99.7%,相对标准偏差为8.1%-15.7%。结论本方法操作简单、快速、灵敏、稳定性好,可作为椰子汁或复原椰子汁中乙基麦芽酚、香兰素、甲基香兰素、乙基香兰素和椰子醛的定量和定性检测方法。Objective To establish a method for the detection of ethyl maltol, vanillin, methyl vanillin, ethyl vanillin and cocoanut aldehyde in coconut milk or reconstituted coconut milk by gas chromatography. Methods Appropriate amount of coconut or reconstituted coconut sample were taken, purified by diatomaceous earth solid phase extraction column, eluted with ethyl acetate as eluent for elution, and detected by gas chromatography, quantified by external standard method. Results Ethyl maltol had a good linear relationship in the range of 5-100 μg/mL(r>0.990), the detection limit was 2 mg/kg, the quantification limit was 6 mg/kg, the recoveries were 88.4%-93.2%, and the relative standard deviations were 2.8%-3.6%;vanillin, methyl vanillin and ethyl vanillin had a good linear relationship in the range of 2-100 μg/mL(r>0.990), the detection limit was 1 mg/kg, the quantification limit was 3 mg/kg, the recoveries were 80.9%-114.4%, and the RSDs were 1.8%-5.9%;cocoanut aldehyde had a good linear relationship in the range of 1-100 μg/m L(r>0.990), the detection limit was 0.5 mg/kg, the quantification limit was 1.5 mg/kg, the recoveries were 86.5%-99.7%, and the RSDs were 8.1%-15.7%. Conclusion This method is simple to operate, rapid, sensitive, and stable, and can be used as a quantitative and qualitative detection method for ethyl maltol, vanillin, methyl vanillin, ethyl vanillin and cocoanut aldehyde in coconut milk or reconstituted coconut milk.

关 键 词：乙基麦芽酚 香兰素 甲基香兰素 乙基香兰素 椰子醛 气相色谱法 

分 类 号：O657.71[理学—分析化学] TS255.44[理学—化学]