乳酸菌高密度培养条件优化研究  

Optimization of high-density culture condition of lactic acid bacteria

作　　者：李玉娥[1] 马玲[1] LI Yu'e;MA Ling(College of Food Science and Technology,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西农业大学食品科学与工程学院,山西晋中030801

出　　处：《中国酿造》2021年第9期150-154,共5页China Brewing

基　　金：山西省重点研发计划项目(201903D221035)。

摘　　要：该试验通过单因素试验和响应面试验探究了乳酸菌高密度培养过程中碳酸钠缓冲盐添加时间、培养pH值、营养因子添加量以及后发酵时间对菌落总数的影响。结果表明,乳酸菌高密度培养的最优条件为接种量3%,培养温度42℃,培养2 h时添加15%碳酸钠缓冲盐,将pH调为6.5,同时添加与原发酵培养基等体积的营养因子(乳清∶番茄汁∶胡萝卜汁∶牛奶=4∶6∶6∶5)后再培养4 h。在此优化条件下,培养液的活菌数较高,为5.68×10^(11) CFU/mL。The effect of sodium carbonate buffer salt addition time,culture pH value,nutrient addition and post-fermentation time on the total number of colonies during high-density culture of lactic acid bacteria was explored through signal factor experiment and response surface methodology.The results showed that the optimal conditions for high-density culture of lactic acid bacteria were inoculum 3%,culture temperature 42℃,adding sodium carbonate buffer salt cultured for 2 h,adjusting pH to 6.5,adding the same volume of nutrient factors(whey∶tomato juice∶carrot juice∶milk=4∶6∶6∶5),and continued culture for 4 h.Under these optimal conditions,the number of viable bacteria in the culture solution was higher,which was 5.68×10^(11)CFU/ml.

关 键 词：乳酸菌 高密度培养 营养因子 活菌数 培养条件优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]