双酶法提取大蒜水溶性膳食纤维及其抗氧化活性分析  

Extraction of Water Soluble Dietary Fiber from Garlic by Double Enzyme Method and Analysis of its Antioxidant Activity

作　　者：张云 苗敬芝[1,2] 董玉玮 李勇[1,2] 邓锦慧 罗姣[1] ZHANG Yun;MIAO Jingzhi;DONG Yuwei;LI Yong;DENG Jinhui;LUO Jiao(School of Food and Biology Engineering,Xuzhou University of Engineering,Xuzhou,Jiangsu 221018,China;Jiangsu Food Resources Development and Quality Safety Key Construction Laboratory,Xuzhou,Jiangsu221018,China)

机构地区：[1]徐州工程学院食品与生物工程学院,江苏徐州221018 [2]江苏省食品资源开发与质量安全重点建设实验室,江苏徐州221018

出　　处：《农产品加工》2021年第18期15-17,21,共4页Farm Products Processing

基　　金：徐州工程学院大学生创新训练计划项目(XCX2020050);徐州市重点研发计划项目(KC18120)。

摘　　要：以大蒜为原料,采用双酶法提取大蒜水溶性膳食纤维,研究纤维素酶加酶量、木瓜蛋白酶加酶量、酶解时间、酶解温度对大蒜水溶性膳食纤维得率的影响,并对其抗氧化活性进行分析。结果表明,大蒜水溶性膳食纤维最佳提取条件为纤维素酶添加量2.2%,木瓜蛋白酶添加量2.2%,酶解时间180 min,酶解温度55℃,大蒜水溶性膳食纤维得率32.06%。大蒜水溶性膳食纤维对对O2-自由基和DPPH自由基均表现出较强的清除能力,且随其质量浓度的增加清除率增大,呈良好的量效关系。因此,大蒜水溶性膳食纤维是一种优质的食物纤维,可作为食品添加剂应用。In this experiment,garlic was used as raw material to extract water-soluble dietary fiber from garlic by double enzyme method.The effects of cellulase dosage,papain dosage,enzymolysis time and enzymolysis temperature on the yield of garlic water-soluble dietary fiber were studied,and its antioxidant activity was analyzed.The results revealed that the optimum extraction conditions were cellulase 2.2%,papain 2.2%,enzymolysis time 180 min,enzymolysis temperature 55℃,and the yield of water-soluble dietary fiber was 32.06%.Garlic water soluble dietary fiber showed a strong scavenging ability to O2-free radical,DPPH free radical,and the scavenging rate increased with the increase of its concentration,showing a good dose effect relationship.Therefore,it was a kind of high quality food fiber and can be used as food additive.

关 键 词：大蒜 水溶性膳食纤维 双酶法 正交试验 抗氧化活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]