绣球菌中抑制α-葡萄糖苷酶活性组分的筛选及作用机理  

Screening and Mechanism of Inhibitingα-Glucosidase Activity Fraction from Sparassis crispa

作　　者：吴勇 王泽玉 周娜 赵福 赵佳山 李耘业 王欣 李金杰[1] WU Yong;WANG Zeyu;ZHOU Na;ZHAO Fu;ZHAO Jiashan;LI Yunye;WANG Xin;LI Jinjie(Beijing Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing 100191,China)

机构地区：[1]北京联合大学,生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《食品工业科技》2021年第22期93-98,共6页Science and Technology of Food Industry

基　　金：北京市教育委员会科学研究计划项目(KM202011417014)。

摘　　要：在体外抑制α-葡萄糖苷酶活性指导下,探究绣球菌中不同极性组分对α-葡萄糖苷酶的抑制作用及机理。采用两种不同乙醇浓度对绣球菌进行超声辅助提取,提取液经萃取、大孔吸附树脂初步纯化后,获得不同极性组分。将上述所有组分进行体外α-葡萄糖苷酶抑制活性筛选,获得抑制活性组分,计算半数抑制浓度(Half maximal inhibitory concentration,IC50),并用酶促动力学和Lineweaver-Burk双倒数法探讨作用机制。结果表明,在所有活性筛选组分中,用50%乙醇提取,大孔吸附树脂60%乙醇洗脱组分抑制α-葡萄糖苷酶的活性最强,其IC50为0.0927±0.0600 mg/mL,与阿卡波糖(IC50:0.0795±0.0200 mg/mL)活性相当,是竞争与非竞争的混合型抑制类型。此研究明确了绣球菌中抑制α-葡萄糖苷酶活性的主要组分,并探讨了作用机制,为进一步利用绣球菌开发食用降糖产品提供了理论科学依据。Under the guidance of inhibitionα-glucosidase activity in vitro,the inhibition effect and mechanism of inhibitingα-glucosidase activity of different polar components obtained from Sparassis crispa were investigated.The extracts of S.crispa were obtained by the ultrasonic-assisted extraction methods with two different ethanol concentrations.The different polar components were obtained by the extraction and the macroporous adsorption resin.All of these fractions were screened by theα-glucosidase inhibitory activity in vitro,and the active fractions were obtained.Half maximal inhibitory concentration(IC50)was calculated,and the mechanism of action was investigated by enzymatic kinetics and Lineweaver-Burk methods.The results showed that the inhibition ofα-glucosidase activity was strongest in the 60%ethanol eluting fraction of the extract of 50%ethanol,with IC50 value of 0.0927±0.0600 mg/mL,which was equivalent to the activity of acarbose(IC50:0.0795±0.0200 mg/mL).It was competitive and non-competitive mixed inhibition type.This study identified the avtivity fraction of inhibitingα-glucosidase in the extract of S.crispa,and discussed the mechanism of action.It would provide a theoretical and scientific basis for developing hypoglycemic products for S.crispa in the future.

关 键 词：绣球菌 Α-葡萄糖苷酶 提取 分离纯化 大孔吸附树脂 

分 类 号：TS201.4[轻工技术与工程—食品科学]