响应面分析法优化黑松松花粉多糖提取工艺  

Optimization of extraction process of black pine pollen polysaccharide by response surface analysis

作　　者：王双毅 谢玮[1] 崔少宁[1] 葛胜菊 肖川[1] WANG Shuang-yi;XIE Wei;CUI Shao-ning;GE Sheng-ju;XIAO Chuan(Department of Food,Yantai Nanshan University,Yantai 265713,Shandong,China)

机构地区：[1]烟台南山学院食品系,山东烟台265713

出　　处：《粮食与油脂》2021年第11期113-116,120,共5页Cereals & Oils

基　　金：2021年第一批产学合作协同育人项目(202101055002、202101055026);2020烟台南山学院校级教改项目“产教融合背景下校企协同“教-学-研-赛”教学模式研究——以《肉制品加工工艺学》为例”;2019山东省自然科学基金项目(ZR2019PC006);2021年烟台社科联项目(YTSK2021-111)。

摘　　要：以黑松松花粉为原料,采用超声波和纤维素酶辅助提取松花粉多糖。在单因素试验的基础上采用响应面分析方法,得到提取工艺的最优条件。结果表明:影响松花粉多糖得率的因素按主次顺序分别为水浴时间>酶解p H>纤维素酶添加量>水浴温度,多糖提取的最佳工艺条件为纤维素酶添加量1.5%(以松花粉质量为基准)、水浴温度51℃、酶解p H 6.0、水浴时间50 min,在此条件下松花粉多糖得率预测值为2.08%,验证值为2.00%。The extraction of pine pollen polysaccharides was performed with the aid of ultrasonic and cellulase using black pine pollen as raw material.On the basis of single factor experiment,response surface method(RSM)was used to optimized the conditions of extraction process.The results showed that the factors influencing the polysaccharide yield of pine pollen were water bath time>enzymatic p H>cellulase addition>water bath temperature,and the optimal conditions for polysaccharide extraction were cellulase addition 1.5%(based on the mass of pine pollen),water bath temperature 51℃,enzymatic p H 6.0,water bath time 50 min,under these conditions,the predicted value of pine pollen polysaccharide yield was 2.08%and the validated value was 2.00%.

关 键 词：松花粉多糖 纤维素酶 超声波 响应面分析法 

分 类 号：TS201.1[轻工技术与工程—食品科学]