基于LAMP技术检测速冻肉糜类制品中单增李斯特菌的增菌方法研究及应用  

Study on the Enrichment Methods and Application of LAMP Technology for Detection of Listeria monocytogenes in Frozen Meat Products

作　　者：岳慧敏 李海鑫 罗瑞平 赵亮[1,2,4] YUE Huimin;LI Haixin;LUO Ruiping;ZHAO Liang(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Xinghua Industrial Research Centre for Food Science and Human Health,China Agricultural University,Xinghua 225700,China;Taizhou Anjoy Foods Co.,Ltd.,Xinghua 225700,China;Beijing Laboratory for Food Quality and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业大学(兴化)健康食品产业研究院,江苏兴化225700 [3]泰州安井食品有限公司,江苏兴化225700 [4]食品质量安全北京实验室,食品科学与营养工程学院,中国农业大学,北京100083

出　　处：《食品工业科技》2021年第24期128-135,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金(32072196,31901625);生猪产业技术体系北京市创新团队(BAIC-2020)。

摘　　要：本研究比较四种增菌培养基(Fraser、Half Fraser、LB_(1)、LB_(2))对速冻肉糜类制品中单增李斯特菌的增殖效果,评价其对速冻肉糜类制品中单增李斯特菌LAMP检测检出限的影响,并将优化后的增菌条件结合LAMP技术应用于人工污染样本和实际生产线样本检测。结果显示,四种增菌培养基中Half Fraser的增菌效果最好,在6 h内实现单增李斯特菌活菌数从6.4×10^(1) CFU/mL增菌至9.4×10^(4) CFU/mL。结合优化增菌条件后,LAMP检测速冻肉糜类制品中单增李斯特菌的检出限从6.4×10^(4) CFU/g(未增菌)降低至6.4×10^(1) CFU/g。利用人工污染单盲样本以及生产线实际样本验证方法的可靠性,结果显示增菌结合LAMP方法与国标法结果一致,准确度100%。该方法样本处理、检测、结果判定总时间控制在8 h内,能够满足企业现场检测需要。In this study efficiency of four enrichment media(Fraser,Half Fraser,LB_(1),LB_(2))were evaluated for Listeria monocytogenes in frozen meat products,and their effects on the detection limit of Listeria monocytogenes by LAMP were assessed in frozen meat products.Combined with LAMP technology,the optimized method was applied to the detection of artificial contamination samples and samples from production line.The results showed that Half Fraser represented the best enrichment among the four media,which could increase the number of live Listeria monocytogenes from 6.4×10^(1)CFU/mL to 9.4×10^(4) CFU/mL within 6 h.The detection limit of Listeria monocytogenes in frozen meat products reduced from 6.4×10^(4) CFU/g(without enrichment)to 6.4×10^(1) CFU/g by LAMP with Half Fraser-enrichment.The reliability of the method was verified by using artificial contamination samples and actual samples from production line of the plant.The results obtained from Half Fraser-LAMP were consistent with the results from national standard method with an accuracy of 100%.The whole procedure of sample processing and testing was within 8 h,which could meet the on-site testing requirements of enterprises.

关 键 词：单核细胞增生李斯特氏菌 速冻肉糜类制品 增菌培养 环介导等温扩增(LAMP) 

分 类 号：TS207.4[轻工技术与工程—食品科学]