水溶性壳聚糖对3种微生物的抑制作用差异  

Difference of Inhibition Effects of Water Soluble Chitosan on Three Kinds of Microorganisms

作　　者：王向阳[1] 卢进彬 潘炎[1] 顾双[1] Wang Xiangyang;Lu Jinbin;Pan Yan;Gu Shuang(Collage of Food and Bioengineering,Zhejiang Gongshang University,Hangzhou 310018)

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310018

出　　处：《中国食品学报》2021年第11期53-61,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省公益技术研究计划农业项目(LGN18C200014)。

摘　　要：目的:研究壳聚糖对不同微生物的抑制作用差异。方法:使用水溶性壳聚糖处理乳酸菌、大肠杆菌和酵母菌,测定壳聚糖对3种菌的最低抑制浓度(MIC)和最低杀菌浓度(MBC),测定3种菌的相对电导率、溶出OD_(260nm)值、脱氢酶、β-半乳糖苷酶活性。结果:壳聚糖对乳酸菌、大肠杆菌、酵母菌的MIC分别为0.2,2,0.4 mg/L,MBC分别为0.8,16,3.2 mg/L。4MIC壳聚糖显著提高所有菌的相对电导率和OD_(260nm)值。MIC壳聚糖处理引起所有菌相对电导率和酵母菌OD_(260nm)值显著上升和大肠杆菌OD_(260nm)值下降;且乳酸菌OD_(260nm)值在培养0.5 h后无显著差异,培养9 h后显著上升。壳聚糖显著抑制乳酸菌和酵母菌的脱氢酶活性,然而对大肠杆菌无显著抑制。壳聚糖对β-半乳糖苷酶活性的抑制较脱氢酶弱。结论:壳聚糖易穿透乳酸菌的细胞壁,也易穿越酵母菌的细胞壁并破坏细胞膜,而穿透大肠杆菌的细胞壁较难。壳聚糖抑菌性差异可能与细胞壁对壳聚糖阻隔和抵抗细胞膜破坏能力有关。Objectives:In order to investigate the mechanism of different inhibitory effects of chitosan on some microorganisms.Methods:The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of chitosan were determined,and the relative conductivity,dissolved OD_(260nm)value,dehydrogenase andβ-galactosidase activity were measured.Results:The results showed that the MIC of chitosan to Lactobacillus plantarum,Escherichia coli and Saccharomyces ellipsoideus were 0.2,2,0.4 mg/L,MBC were 0.8,16 mg/L and 3.2 mg/L,respectively.4MIC chitosan significantly increased the relative conductivity and OD_(260nm)value of all microorganisms.MIC chitosan treatments increased the relative conductivity of all microorganisms and OD_(260nm)value of S.ellipsoideus significantly,but the OD_(260nm)value of E.coli decreased,and no significant difference was found in L.plantarum at 0.5 h,but its increased significantly at 9h.Chitosan significantly inhibited the dehydrogenase of L.plantarum and S.ellipsoideus,but had no significant effect on E.coli.The inhibition effect of chitosan onβ-galactosidase was weaker than that of dehydrogenase.Conclusions:Chitosan was easy to pass through the cell wall of L.plantarum and S.ellipsoideus,also damage cell membrane of S.ellipsoideus.But it was difficult to pass through E.coli cell wall.The difference of antibacterial activity of chitosan might be related to the barrier ability of cell wall and resisting destruction of cell membrane to chitosan.

关 键 词：壳聚糖 葡萄酒酵母 植物乳杆菌 大肠杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]