草鱼鳞酶解物的制备及其促嗜热链球菌生长作用  

Preparation of grass fish scale hydrolysate and its proliferation effect on Streptococcus thermophiles growth

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作  者:李路瑶 曾丽芳 宋益善[1,2] 陈建康 LI Luyao;ZENG Lifang;SONG Yishan;CHEN Jiankang(College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;National R&D Branch Center for Freshwater Aquatic Products Processing Technology,Shanghai 201306,China)

机构地区:[1]上海海洋大学食品学院,上海201306 [2]国家淡水水产品加工技术研发中心,上海201306

出  处:《上海海洋大学学报》2021年第6期1132-1141,共10页Journal of Shanghai Ocean University

基  金:上海海洋大学科技发展专项基金(A2-2006-00-200209);上海市大学生创新基金(2020)。

摘  要:为提高水产品综合利用率和开发新型益生菌功能性食品,以鱼类加工副产物草鱼鳞为研究对象,利用风味蛋白酶对其进行酶解,并以酶解物促嗜热链球菌增殖作用为评价指标,结合单因素试验与响应面Box-Benhnken中心组合试验优化酶解工艺,得到最优酶解条件:酶解时间3.8 h,料液比(w/v)1∶3.2 g/mL,加酶量6.2%。在此条件下,嗜热链球菌增殖量ΔOD_(600)=1.1734±0.0150。同时,将最优酶解条件下得到的酶解物进行膜过滤分离,得到分子量<1 ku、1~<3 ku、3~<5 ku、5~<10 ku、≥10 ku的5个组分,并进一步就各组分对嗜热链球菌的生长曲线和产酸能力的影响进行研究。结果表明,在嗜热链球菌24 h的生长曲线中,分子量<1 ku的组分促嗜热链球菌生产和产酸效果最好:嗜热链球菌增殖量ΔOD_(600)为1.050,培养基的pH比初始值降低了1.06。In order to improve the comprehensive utilization of aquatic products and develop new functional foods containing probiotic bacteria,by-product of fish processing-the grass fish scales was studied.The grass fish scales were hydrolyzed by flavourzyme,and the enzymatic hydrolysis process was optimized by the single factor experiments and the response surface methodology(RSM) using the proliferation of Streptococcus thermophilus as the evaluation index.The results showed that the optimum conditions were the reaction time 3.8 h,the ratio of material to water 1∶3.2 g/mL and the concentration of enzyme 6.2%.Under these conditions,the ΔOD_(600) of proliferated Streptococcus thermophilus was 1.173 4±0.015 0.Meantime,the GFSH under the optimum conditions was further separated into 5 segments by membrane filtration,which were:<1 ku,1-<3 ku,3-<5 ku,5-<10 ku,≥10 ku.The effects of five segments on the growth curve and production of acid of Streptococcus thermophilus were studied.The results showed that in the 24-hour growth period of Streptococcus thermophilus,the fragments with molecular weights of less than 1 000 u significantly promoted the growth and acid production of Streptococcus thermophilus,and the ΔOD_(600) of proliferated Streptococcus thermophilus was 1.050,and the pH of the medium was reduced by 1.06.

关 键 词:草鱼鳞 酶解 嗜热链球菌 增殖 

分 类 号:TS254.1[轻工技术与工程—水产品加工及贮藏工程]

 

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