聚乙二醇1000维生素E琥珀酸酯-皂皮皂素微乳液的制备、表征及活性  

Preparation,Characterization and Activity of D-α-Tocopheryl Polyethylene Glycol 1000 Succinate-Saponin Microemulsion

作　　者：吕奇晏 林诗叶 米亚妮 钱跃威 滕慧[1] 陈雷 LÜQiyan;LIN Shiye;MI Yani;QIAN Yuewei;TENG Hui;CHEN Lei(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524025,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]广东海洋大学食品科技学院,广东湛江524025

出　　处：《食品科学》2021年第24期9-15,共7页Food Science

基　　金：国家自然科学基金面上项目(32072209)。

摘　　要：选用毒性小、刺激性低且有P-糖蛋白抑制剂作用的聚乙二醇1000维生素E琥珀酸酯(D-α-tocopheryl polyethylene glycol 1000 succinate,TPGS)和乳化效果强的天然植物化合物皂皮皂素(quillaja saponin,QS),与聚氧乙烯氢化蓖麻油(RH40)复配为乳化剂,再以油酸乙酯为油相,异丙醇为助乳化剂,配制TPGS-QS微乳液。利用普通光学显微镜、透射电子显微镜和激光粒度测定仪观察微乳液液滴形貌、粒径分布和Zeta电位。结果:TPGS-QS微乳液具体配制方法为m(油酸乙酯)∶m(质量分数0.02%TPGS溶液)∶m(质量分数1.5%QS溶液)∶m(RH40)∶m(异丙醇)=30∶6.6∶6.6∶39.4∶17.5,所用溶液均以纯水配制。该微乳液外观淡黄、澄清、透明,流动性强,液滴呈均一规则的圆球形,为O/W型乳液,微乳液平均粒径为(48.89±0.08)nm,Zeta电位为(-4.513±0.564)mV。为验证TPGS-QS微乳液是否具有生物活性,测定其α-葡萄糖苷酶抑制活性、1,1-二苯基-2-三硝基苯肼自由基清除能力、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力、铁离子还原能力,及其对HepG2和Caco-2细胞的毒性作用,发现该微乳液对α-葡萄糖苷酶活性的半抑制质量浓度为67.15 mg/mL,具有一定的抗氧化活性,并且几乎不会影响细胞的正常生长,甚至在一定质量浓度范围对细胞有增殖效果。D-α-tocopheryl polyethylene glycol 1000 succinate(TPGS),an inhibitor of P-glycoprotein(P-gp)with low toxicity and irritation,and the natural phytochemical quillaja saponin(QS),with a strong emulsifying effect,were selected for mixing with ethoxylated hydrogenated castor oil(RH40)as emulsifiers to prepare TPGS-QS microemulsion with ethyl oleate as an oil phase and isopropanol as a co-emulsifier.The morphology,particle size and zeta potential of emulsion droplets were observed by optical microscopy(OM),transmission electron microscopy(TEM)and laser particle size analyzer.The optimal microemulsion formulation consisted of a mixture of ethyl oleate,0.02%TPGS,1.5%QS,RH40 and isopropanol at a ratio of 30:6.6:6.6:39.4:17.5(m/m).All solutions were prepared with purified water.The microemulsion was yellowish,clear and transparent in appearance with strong fluidity,and it was identified as an O/W-type emulsion with uniform spherical droplets.In addition,its average particle size was(48.89±0.08)nm,and its zeta potential was(–4.513±0.564)mV.Theα-glycosidase inhibitory activity,1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacity,2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate)(ABTS)cation radical scavenging capacity and iron reducing power of TPGS-QS microemulsion was tested,and its in vitro cytotoxicity on HepG2 and Caco-2 cells was evaluated through MTT assay.The half-maximal inhibitory concentration againstα-glycosidase was 67.15 mg/mL,and the emulsion had antioxidant activity.It hardly affected the normal growth of cells,but even had a promoting effect on cell proliferation within a certain concentration range.

关 键 词：聚乙二醇1000维生素E琥珀酸酯 皂皮皂素 微乳液 抗氧化性 细胞毒性 

分 类 号：TS201.4[轻工技术与工程—食品科学]