H_(2)O_(2)-VC降解对香水莲花多糖结构与活性的影响  

Effect of H_(2)O_(2)-VC Degradation System on the Structure and Activity of Polysaccharides from Nymphaea hybrid

作　　者：谭诗敏 罗志刚[1] 程建华 TAN Shimin;LUO Zhigang;CHENG Jianhua(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China;School of Environment and Energy,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640 [2]华南理工大学环境与能源学院,广东广州510640

出　　处：《食品科学》2021年第24期48-53,共6页Food Science

基　　金：广东省东莞市社会科技发展项目(2019507163434);中央高校基本科研业务费专项(D2192900)。

摘　　要：采用超声辅助热水提取香水莲花多糖(Nymphaea hybrid polysaccharides,NHP),并经H_(2)O_(2)-VC体系降解制备3种低分子质量多糖:DNHP-1、DNHP-2、DNHP-3。同时,进一步研究NHP和DNHP的结构、流变性、功能特性及抗氧化活性。结果表明:NHP降解后半乳糖醛酸、甘露糖含量下降,鼠李糖含量增加,但该多糖的单糖种类未改变。红外光谱也表明降解未破坏多糖的主要官能团结构。刚果红实验证明4种多糖均不具有三股螺旋结构。流变性实验表明NHP降解后降低了多糖的表观黏度,增加了多糖的固体弹性。功能特性实验表明NHP降解后持油性、乳化性降低,持水性增加。1,1-二苯基-2-三硝基苯肼自由基、2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐阳离子自由基、羟自由基清除实验表明,降解时间为60 min的DNHP-2抗氧化活性更高。因此,本研究为NHP在食品、化妆品、药品等领域的应用提供理论依据。Polysaccharides(NHP)extracted from Nymphaea hybrid by ultrasonic-assisted hot water extraction were degraded with a combination of ascorbic acid and H_(2)O_(2)into three low-molecular-mass polysaccharides(DNHP-1,DNHP-2 and DNHP-3).The structural,rheological,functional and antioxidant properties of the polysaccharides were studied.The results showed that although the monosaccharide types of NHP kept unchanged after degradation,the molar percentages of galacturonic acid and mannose decreased and the molar percentage of rhamnose increased.Fourier transform infrared(FTIR)spectroscopic analysis revealed that the primary structures of the polysaccharides were not affected after degradation.Congo red test showed that none of the four polysaccharides had triple helix structures.Rheological analysis demonstrated that DNHP solutions displayed lower apparent viscosity than NHP solution at the same concentration,while the elasticity of DNHP was improved compared with that of NHP.DNHP had better water-holding capacity and weaker oil-holding and emulsifying capacity.Furthermore,DNHP-2 collected after 60 minutes of degradation had stronger antioxidant activity in terms of scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl(DPPH)radicals,2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid(ABTS)radical cation and hydroxyl radicals.The results of this study may provide a theoretical basis for the application of N.hybrid polysaccharides in foods,cosmetics and medicines.

关 键 词：香水莲花 多糖 降解 低分子多糖 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]