秀珍菇多酚提取工艺优化及抗氧化活性探究  

Optimization on Extraction Technology of Polyphenols from Pleurotus geesteranus and Study on the Antioxidant Activity

作　　者：杨斌 宋厚辑 郑峻 夏俊慧 李佳欢[1,2] 兰世步 孙淑静 胡开辉[1,2] 金文松 YANG Bin;SONG Hou-ji;ZHENG Jun;XIA Jun-hui;LI Jia-huan;LAN Shi-bu;SUN Shu-jing;HU Kai-hui;JIN Wen-song(College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fungi Research Institute(Gutian)of Fujian Agriculture and Forestry University,Ningde 352200,China;Fujian Edible Fungi Technical Popularization Station,Fuzhou 350003,China;Edible Fungi Research and Development Center in Luoyuan County,Fuzhou 350600,China)

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学(古田)菌业研究院,福建宁德352200 [3]福建省食用菌技术推广总站,福州350003 [4]罗源县食用菌研发中心,福州350600

出　　处：《中国调味品》2022年第1期183-189,共7页China Condiment

基　　金：国家自然科学基金(81803667);中国博士后科学基金面上项目(132300346);福建省科技厅对外产业化合作项目(2020I1008);福建农林大学科技创新专项基金(114/KFA17322A)。

摘　　要：目的:应用响应面设计优化超声辅助水提秀珍菇多酚工艺,分析多酚的抗氧化能力。方法:应用单因素与Box-Behnken设计,优化秀珍菇多酚提取工艺,并建立多酚体外总抗氧化分析与DPPH、ABTS自由基清除分析。结果:优化设计实验结果表明秀珍菇多酚最佳提取工艺为:液料比68∶1(mL/g),超声功率300 W,提取时间87 min,提取温度45℃,多酚得率为(17.428±0.121)mg/g。体外抗氧化分析结果表明,秀珍菇多酚总抗氧化能力EC_(50)=0.177 mg/mL,DPPH自由基清除率与ABTS自由基清除率IC_(50)分别为0.363 mg/mL与0.008 mg/mL。结论:秀珍菇多酚的提取工艺可行,秀珍菇多酚具有较强的抗氧化能力。Objective:Response surface design(RSM)is used to optimize the ultrasound-assisted water extraction process of polyphenols from Pleurotus geesteranus and to analyze the antioxidant capacity of polyphenols.Methods:Single factor and Box-Behnken design are used to optimize the extraction process of polyphenols from Pleurotus geesteranus,and total antioxidant analysis and DPPH and ABTS radicals scavenging analysis of polyphenols in vitro are established.Results:The optimal extraction conditions are as follows:liquid-solid ratio is 68∶1(mL/g),ultrasonic power is 300 W,extraction time is 87 min,extraction temperature is 45℃,and the yield of polyphenols is(17.428±0.121)mg/g.The results of antioxidant analysis in vitro show that the total antioxidant capacity EC_(50) is 0.177 mg/mL,the IC_(50) of DPPH radical scavenging rate and ABTS radical scavenging rate is 0.363 mg/mL and 0.008 mg/mL respectively.Conclusion:The extraction technology of polyphenols from Pleurotus geesteranus is feasible,and polyphenols from Pleurotus geesteranus have strong antioxidant ability.

关 键 词：秀珍菇 多酚提取 响应面设计 抗氧化活性 

分 类 号：TS202.3[轻工技术与工程—食品科学]