刺梨果酒对高脂诱导肥胖小鼠脂代谢的影响  

Effect of Rosa roxburghii Wine on Lipid Metabolism Disorders in High Fat-induced Obese Mice

作　　者：陈珍 陆敏涛 徐方艳 邵林子 任廷远 谭书明 CHEN Zhen;LU Mintao;XU Fangyan;SHAO Linzi;REN Tingyuan;TAN Shuming(School of Wine and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Guizhou Province Agricultural Bioengineering Key Laboratory,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州省农业科学院,贵州贵阳550006 [3]贵州省农业生物工程重点实验室,贵州贵阳550025

出　　处：《食品工业科技》2022年第3期358-366,共9页Science and Technology of Food Industry

基　　金：贵州省林业局科技项目,黔林科合[2020]2015;贵州省科技厅项目,黔科合平台人才[2020]2105;贵州省工业和信息化厅2020年刺梨产业专项,黔财农[2020]307号;贵州省林业局科技项目,特林研2020-010。

摘　　要：目的:研究刺梨果酒对高脂诱导小鼠肥胖发生过程预防效果及其机理。方法:将50只小鼠随机分为空白组、模型组、刺梨果酒低剂量组(0.25 mL/80 g)、中剂量组(0.5 mL/80 g)和高剂量组(1 mL/80 g),每组10只,实验时间8周。实验结束后,测定小鼠脏器指数、血清及肝脏脂代谢相关生理生化指标;应用qRT-PCR测定肝脏过氧化物酶体增殖物激活受体α(Peroxisome proliferators-activated receptor-α,PPARα)、固醇调节元件结合蛋白(Sterol-regulatory element binding proteins,SREBP1)、硬脂酰辅酶A去饱和酶(Stearyl-coenzyme A dehydrogenase-1,SCD1)、乙酰辅酶A羧化酶(Acetyl-CoA carboxylases alpha,ACACA)、脂肪酸合成酶(Fatty acid synthase,FASN)、肝X受体(Liver X receptor,LXR)、腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)mRNA相对表达量。结果:与模型组相比,刺梨果酒可显著(P<0.05)减缓小鼠体重增加,降低脂肪指数及血清和肝脏中总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、低密度脂蛋白胆固醇(Low density lipoprotein cholesterol,LDL-C)的含量,升高高密度脂蛋白胆固醇(Hight density lipoprotein cholesterol,HDL-C)含量。此外,高剂量组可显著(P<0.05)下调小鼠肝脏中PPARα、AMPK、LXR、ACACA、SCD1、FASN基因表达水平。HE染色结果显示,剂量组能缓解肝细胞肿大,减少肝脏脂肪变性。结论:刺梨果酒对高脂诱导小鼠肥胖具有预防作用,可能与减少体内脂肪堆积,改善脂代谢紊乱有关。Objective:To study the preventive effect and its mechanism of Rosa roxburghii wine on obesity process in high fat-induced mice.Method:50 mice were randomly divided into a blank group,a model group,a low-dose(0.25 mL/80 g),a medium-dose(0.5 mL/80 g)and a high-dose Rosa roxburghii wine group(1 mL/80 g),each group of 10 mice,the experiment was carried out for 8 weeks.After the experiment,the viscera coefficient,serum and liver lipid metabolismrelated physiological and biochemical indicators were measured.The mRNA relative expression of PPARα(Peroxisome proliferators-activated receptor-α),SREBP1(Sterol-regulatory element binding proteins),SCD1(Stearyl-coenzyme A dehydrogenase-1),ACACA(Acetyl-CoA carboxylases alpha),FASN(Fatty acid synthase),LXR(Liver X receptor)and AMPK(AMP-activated protein kinase)were measured by qRT-PCR.Results:Compared with model group,Rosa roxburghii wine could significantly(P<0.05)slow down the weight gain of mice,reduce fat index and total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)content in serum and liver,and increase the content of high density lipoprotein cholesterol(HDL-C).In addition,the high-dose group significantly down-regulated the mRNA relative expressions of PPARα,AMPK,LXR,ACACA,SCD1 and FASN.The HE staining results showed that Rosa roxburghii wine could relieve hepatocellular enlargement and reduce hepatic steatosis.Conclusion:Rosa roxburghii wine has a preventive effect on high-fat-induced obesity in mice,which may be attributed to its ability to reduce fat accumulation and improve lipid metabolism disorders.

关 键 词：刺梨果酒 高脂饮食 肥胖 脂质代谢 

分 类 号：TS201.4[轻工技术与工程—食品科学]